Chimeric influenza-virus-like particles containing the porcine reproductive and respiratory syndrome virus GP5 protein and the influenza virus HA and M1 proteins

Chimeric influenza-virus-like particles containing the porcine reproductive and respiratory... Both porcine reproductive and respiratory syndrome and swine influenza are acute, highly contagious swine diseases. These diseases pose severe threats for the swine industry and cause heavy economic losses worldwide. In this study, we have developed a chimeric virus-like particle (VLP) vaccine candidate for porcine reproductive and respiratory syndrome virus (PRRSV) and H3N2 influenza virus and investigated its immunogenicity in mice. The HA and M1 proteins from the H3N2 influenza virus and the PRRSV GP5 protein fused to the cytoplasmic and transmembrane domains of the NA protein were both incorporated into the chimeric VLPs. Analysis of the immune responses showed that the chimeric VLPs elicited serum antibodies specific for both PRRSV GP5 and the H3N2 HA protein, and they stimulated cellular immune responses compared to the responses to equivalent amounts of inactivated viruses. Taken together, the results suggested that the chimeric VLP vaccine represents a potential strategy for the development of a safe and effective vaccine to control PRRSV and H3N2 influenza virus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Chimeric influenza-virus-like particles containing the porcine reproductive and respiratory syndrome virus GP5 protein and the influenza virus HA and M1 proteins

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Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-014-2178-0
Publisher site
See Article on Publisher Site

Abstract

Both porcine reproductive and respiratory syndrome and swine influenza are acute, highly contagious swine diseases. These diseases pose severe threats for the swine industry and cause heavy economic losses worldwide. In this study, we have developed a chimeric virus-like particle (VLP) vaccine candidate for porcine reproductive and respiratory syndrome virus (PRRSV) and H3N2 influenza virus and investigated its immunogenicity in mice. The HA and M1 proteins from the H3N2 influenza virus and the PRRSV GP5 protein fused to the cytoplasmic and transmembrane domains of the NA protein were both incorporated into the chimeric VLPs. Analysis of the immune responses showed that the chimeric VLPs elicited serum antibodies specific for both PRRSV GP5 and the H3N2 HA protein, and they stimulated cellular immune responses compared to the responses to equivalent amounts of inactivated viruses. Taken together, the results suggested that the chimeric VLP vaccine represents a potential strategy for the development of a safe and effective vaccine to control PRRSV and H3N2 influenza virus.

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 2014

References

  • Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview
    Albina, E
  • Efficient induction of mucosal and systemic immune responses by virus-like particles administered intranasally: implications for vaccine design
    Bessa, J; Schmitz, N; Hinton, HJ; Schwarz, K; Jegerlehner, A; Bachmann, MF
  • Influenza vaccines based on virus-like particles
    Kang, SM; Song, JM; Quan, FS; Compans, RW
  • Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus
    Koho, T; Huhti, L; Blazevic, V; Nurminen, K; Butcher, SJ; Laurinmaki, P; Kalkkinen, N; Ronnholm, G; Vesikari, T; Hytonen, VP; Kulomaa, MS
  • Genomic analysis of two Chinese strains of porcine reproductive and respiratory syndrome viruses with different virulence
    Li, Y; Xue, C; Wang, L; Chen, X; Chen, F; Cao, Y
  • Clinical signs and economic losses caused by porcine reproductive and respiratory syndrome virus in a large breeding farm
    Pejsak, Z; Stadejek, T; Markowska-Daniel, I

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