2018 Springer Science+Business Media, LLC
Chemistry of Natural Compounds, Vol. 54, No. 3, May, 2018
CHEMICAL COMPOSITION OF ESSENTIAL OIL
FROM Dionysia hissarica
N. Z. Mamadalieva,
O. T. Turginov,
Sh. S. Azimova,
K. Sh. Tozhibaev,
and S. Bohmdorfer
The genus Dionysia Fenzl (Primulaceae) numbers 49 species that are broadly distributed in Afghanistan, Central
Asia, and Iran. Five species occur in Central Asia; only one, D. hissarica, in the flora of Uzbekistan .
D. hissarica Lipsky from the Khursang River valley of Sangardak basin (1,200–1,600 m above sea level) was first
described in 1901. The plant is a very rare endemic and is currently listed in the Red Book of Uzbekistan as a threatened
species . D. hissarica is a very aromatic plant with a specific aroma. Its chemical composition has not been studied until
now. Herein, essential oil from the aerial part of D. hissarica was analyzed using GC-MS.
The aerial part of D. hissarica was collected in summer 2011 in the Machai Baisun-Tau River valley at 1,600 m above
sea level (Hissar Mountains, Kashkadarya District, Uzbekistan). Taxonomic identification was made at the Botany Institute,
AS, RU. A specimen of the collected D. hissarica is preserved in the herbarium collection under code No. 0251, 9.VII 2011.
Essential oils were produced according to the protocol published in our previous articles [3, 4]. For this, aerial part
of D. hissarica (50 g) was placed into a 500-mL working flask and treated with distilled H
O (~200 mL). Distillate was
collected for 2 h using a Clevenger apparatus and extracted with CH
. The extract of essential oil was dried over anhydrous
. The resulting oil (yield ~0.7%) was stored in a refrigerator at –4°C until use.
Essential-oil constituents were analyzed by GC-MS on an Agilent 7890B gas chromatograph with a 5977A quadrupole
mass spectrometer as the detector, an autosampler, VF-Wax ms quartz column (30 m, 100% polyethylene glycol) with inner
diameter 0.25 Pm (Agilent Technologies, Netherlands), and He carrier gas at constant flow rate 0.9 mL/min. The vaporizer
temperature was 280°C, ion-source temperature 230°C, GC-MS interface temperature 280°C. Electron-impact ionization at
70 eV was used. Data were collected in the mass range 45–950 amu.
Essential oil dissolved in CH
(0.5 PL) was injected automatically with flow division 1:20 into the vaporizer. The
column temperature was maintained at 50°C for 5 min, raised from 50 to 280°C at 5°C/min, and held at 280°C for 15 min at the
end. The injector temperature was 250°C; detector temperature, 270°C. Enhanced ChemStation version MSD F.01.01.2317
software (Agilent Technologies) was used to record and integrate chromatograms. Quantitative contents of essential-oil
constituents were calculated from peak areas. Qualitative analysis was based on comparing retention indices (RI) and total
mass spectra with the corresponding data for standard oil constituents and pure compounds and with GC-MS libraries and the
Wiley Registry of Mass Spectral Data (9
Ed.), NIST Mass Spectral Library (2011), and catalogs [5, 6].
Table 1 lists the essential-oil composition of D. hissarica. A total of 74 constituents were found in the essential oil.
The total content of identified constituents was 93.9%. The main constituents were 2c-hydroxy-5c-methoxyacetophenone
(9.7%), myrtenol (6.9%), globulol (6.4%), eudesm-6-en-4-
-ol (6.0%), 2c-hydroxy-4c-methoxyacetophenone (5.8%),
-cadinol (3.4%), and benzaldehyde dimethyl acetal (3.3%).
1) S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan,
100170, Tashkent, Uzbekistan, e-mail: email@example.com; 2) Institute of Botany, Academy of Sciences of the Republic
of Uzbekistan, 100125, Tashkent, Uzbekistan; 3) University of Natural Resources and Life Sciences (BOKU), 1190 Vienna,
Austria; 4) Biology Faculty, National University of Uzbekistan, 100174, Tashkent, Uzbekistan. Translated from Khimiya
Prirodnykh Soedinenii, No. 3, May–June, 2018, pp. 503–504. Original article submitted November 27, 2017.