2018 Springer Science+Business Media, LLC
Chemistry of Natural Compounds, Vol. 54, No. 3, May, 2018
CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY
OF THE ESSENTIAL OIL FROM Salvia kiangsiensis
Shi-Ping Fang, Xiang Xing, Peng-Xiang Lai, and Jian-Jun Huang*
Salvia kiangsiensis C. Y. Wu is an annual herb that is native to Fujian, Hunan, and Jiangxi Provinces in China,
growing in valleys and forests. S. kiangsiensis typically reaches a height of 45 cm, occasionally taller. Inflorescences are 2–6
flowered widely spaced verticillasters in axillary or terminal racemes or panicles, with a 1.2 cm purple corolla .
The essential oil compositions of S. kiangsiensis have not been previously investigated. Herein we report for the first
time the chemical composition of essential oils from the fresh aerial parts of S. kiangsiensis and evaluate its antioxidant
activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay.
The fresh leaves and small twigs of S. kiangsiensis were collected from Fengxin, Jiangxi Province of China in
August 2016. The plant was identified by Associate Prof. Hong Zhao of Marine College, Shandong University (Weihai).
A voucher specimen (No. 10439) was deposited in the Laboratory of Botany of Marine College, Shandong University (Weihai).
The fresh plant material (500 g) was hydrodistilled for 5 h using a Clevenger apparatus to give the essential oil (0.53 g,
0.106%) . The oil was stored at 4qC until analyses.
The oil sample was analyzed using an Agilent 6890 gas chromatograph equipped with flame ionization detector
(FID). Column: capillary column HP-5MS, 30 m u 0.25 mm u 0.25 Pm film thickness. GC oven temperature was kept at 60°C
for 1 min and programmed to 200qC at a rate of 15qC/min kept constant at 200qC for 5 min, and then programmed to 280qC
at a rate of 5qC/min and kept constant at 280qC for 2 min; injector temperature: 250qC. The flow rate of helium as the carrier
gas was set at 1.2 mL/min; injection volume was 0.2 PL.
GC-MS analyses were carried out using a Hewlett Packard 6890 gas chromatograph fitted with an HP-5MS fused
silica column, interfaced with a Hewlett Packard 5975C mass selective detector operated by HP Enhanced ChemStation
software, version A.03.00. GC parameters were the same as above; Mass spectra were recorded at 70 eV. Mass range was from
m/z 50 to 550.
Identification of the compounds was performed by matching their GC Kovats index obtained with reference to n-alkanes
series on an HP-5MS column and by computer matching with NIST 08 Mass spectral Database for GC-MS .
The results from a chemical analysis of the essential oil of S. kiangsiensis are shown in Table 1. Forty-nine compounds,
which represent 95.49% of the essential oil, were identified. The main constituents of the essential oil were epimanool (45.65%),
-cadinene (3.75%), totarol (3.51%), caryophyllene (3.44%), and
Globulol was reported to have strong antifungal activity on the tested fungi Alternaria solani, Fusarium graminearum,
Rhizoctonia solani, and V. pirina . Totarol decreases the plasma levels of estrogens  and effectively reduces pathogenic
hepatic cells . Moreover it also prevents cells from undergoing oxidative stress in vitro . Caryophyllene was shown to be
a selective agonist of cannabinoid receptor type-2 (CB2) and to exert significant cannabimimetic antiinflammatory effects in
mice . Antinociceptive , neuroprotective , anxiolytic and antidepressant , and anti-alcoholism  activity has
The antiradical activity of the essential oil was found using colorimetry of free radicals and was based on the reaction
of DPPH with the antioxidant [13, 14]. Briefly, stock solutions (10 mg/mL) of the essential oil and the synthetic standard
antioxidant BHT were prepared in methanol. Dilutions were made to obtain concentrations ranging from 10 to 1 mg/mL.
Diluted solutions (2 mL each) were mixed with 2 mL of freshly prepared 53.2 Pg/mL DPPH methanol solution and allowed to
stand for 30 min in the dark at room temperature. The absorbance of these solutions was recorded at 517 nm. However, the
essential oil of S. kiangsiensis was weakly active in this test and showed inhibition percentage of about 4.3% of that of BHT.
Marine College, Shandong University, 264209, Weihai, P. R. China, e-mail: firstname.lastname@example.org. Published in
Khimiya Prirodnykh Soedinenii, No. 3, May–June, 2018, pp. 501–502. Original article submitted October 26, 2016.