Chemical characteristics, antioxidant and anticancer potential of sulfated polysaccharides from Chlamydomonas reinhardtii

Chemical characteristics, antioxidant and anticancer potential of sulfated polysaccharides from... The sulfated polysaccharides (SPs) from Chlamydomonas reinhardtii (Cr) were isolated by hot water method using 80% alcohol and semi purified by anion-exchange column chromatography. The chemical analysis of the extract showed 78% carbohydrates, 18% reducing sugars, 60% non-reducing sugars, 2% protein, 33% sulfate, 39% uronic acid, and 4% ash. The elemental analysis of this C. reinhardtii sulfated polysaccharide-enriched extract (Cr-SPs) showed 53% carbon, 8% hydrogen, and 6% nitrogen. FTIR analysis of Cr-SPs showed characteristic bands of sulfated polysaccharides. Further, the Cr-SPs showed significant hydroxyl radical scavenging activity of 22.29–80.9% at 0.01–1 mg mL−1, 38–77% of DPPH radical scavenging activity at 0.01–1 mg mL−1, 9.8–81% ABTS radical scavenging activity, 34.5–67.6% of ferrous chelating ability, and 11.62–75% of total antioxidant capacity. Cr-SPs also showed efficient in vitro anticancer activity. Specifically, they inhibited triple-negative breast cancer cell (MDA-MB-231) proliferation with an IC50 of 172 μg mL−1. Concentration-dependent reduction in the number of colonies formed by MDA-MB-231 cells suggested their potential to inhibit the clonal expansion of the cancer cells. Higher concentrations of crude extract were found to disrupt the microtubule networks in these cells. The cells treated with Cr-SPs eventually underwent apoptosis as evidenced by the formation of characteristic DNA ladder. These results indicate that Cr-SPs find promising opportunities for cancer treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Phycology Springer Journals

Chemical characteristics, antioxidant and anticancer potential of sulfated polysaccharides from Chlamydomonas reinhardtii

Loading next page...
 
/lp/springer_journal/chemical-characteristics-antioxidant-and-anticancer-potential-of-X7TExnfGdn
Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology
ISSN
0921-8971
eISSN
1573-5176
D.O.I.
10.1007/s10811-018-1397-2
Publisher site
See Article on Publisher Site

Abstract

The sulfated polysaccharides (SPs) from Chlamydomonas reinhardtii (Cr) were isolated by hot water method using 80% alcohol and semi purified by anion-exchange column chromatography. The chemical analysis of the extract showed 78% carbohydrates, 18% reducing sugars, 60% non-reducing sugars, 2% protein, 33% sulfate, 39% uronic acid, and 4% ash. The elemental analysis of this C. reinhardtii sulfated polysaccharide-enriched extract (Cr-SPs) showed 53% carbon, 8% hydrogen, and 6% nitrogen. FTIR analysis of Cr-SPs showed characteristic bands of sulfated polysaccharides. Further, the Cr-SPs showed significant hydroxyl radical scavenging activity of 22.29–80.9% at 0.01–1 mg mL−1, 38–77% of DPPH radical scavenging activity at 0.01–1 mg mL−1, 9.8–81% ABTS radical scavenging activity, 34.5–67.6% of ferrous chelating ability, and 11.62–75% of total antioxidant capacity. Cr-SPs also showed efficient in vitro anticancer activity. Specifically, they inhibited triple-negative breast cancer cell (MDA-MB-231) proliferation with an IC50 of 172 μg mL−1. Concentration-dependent reduction in the number of colonies formed by MDA-MB-231 cells suggested their potential to inhibit the clonal expansion of the cancer cells. Higher concentrations of crude extract were found to disrupt the microtubule networks in these cells. The cells treated with Cr-SPs eventually underwent apoptosis as evidenced by the formation of characteristic DNA ladder. These results indicate that Cr-SPs find promising opportunities for cancer treatment.

Journal

Journal of Applied PhycologySpringer Journals

Published: Jan 19, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off