Charge Displacements in Interfacial Layers Containing Reaction Centers

Charge Displacements in Interfacial Layers Containing Reaction Centers Reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides were oriented in phospholipid interfacial layers adsorbed to a Teflon film separating two electrolyte-filled compartments of a Teflon cell. Light-induced voltage changes were measured as a function of time across electrodes immersed in the cell compartments. The experimental system is characterized both experimentally and theoretically to relate the measured signals to the light-induced displacement currents in the reaction centers. Mathematical relations between the measured signals and the distances and geometries of the charge-transfer reactions are derived. At pH 8.0 the reaction centers were found to be oriented with ∼60% of the population oriented with the donor facing the aqueous phase. The density of the reaction centers in the layer was ∼1011 cm−2, which is close to that found in the native system. Reconstitution of the secondary quinone, Q B , in 90% of the RCs was achieved with an ∼100-fold excess of ubiquinone in the vesicle preparation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Charge Displacements in Interfacial Layers Containing Reaction Centers

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Publisher
Springer-Verlag
Copyright
Copyright © Inc. by 1998 Springer-Verlag New York
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s002329900435
Publisher site
See Article on Publisher Site

Abstract

Reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides were oriented in phospholipid interfacial layers adsorbed to a Teflon film separating two electrolyte-filled compartments of a Teflon cell. Light-induced voltage changes were measured as a function of time across electrodes immersed in the cell compartments. The experimental system is characterized both experimentally and theoretically to relate the measured signals to the light-induced displacement currents in the reaction centers. Mathematical relations between the measured signals and the distances and geometries of the charge-transfer reactions are derived. At pH 8.0 the reaction centers were found to be oriented with ∼60% of the population oriented with the donor facing the aqueous phase. The density of the reaction centers in the layer was ∼1011 cm−2, which is close to that found in the native system. Reconstitution of the secondary quinone, Q B , in 90% of the RCs was achieved with an ∼100-fold excess of ubiquinone in the vesicle preparation.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Oct 1, 1998

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