Characterization of whole-cell k+ currents across the plasma membrane of pollen grain and tube protoplasts of Lilium longiflorum

Characterization of whole-cell k+ currents across the plasma membrane of pollen grain and tube... Outward and inward currents, mainly carried by K+, were detected in protoplasts of pollen grains (PG) and pollen tubes (PT) of Lilium longiflorum Thunb. by using the whole-cell configuration of the patch-clamp technique. The outward K+ current $$(I_{K^ + out} )$$ was similar in both protoplast types, while the inward K+ current $$(I_{K^ + in} )$$ was higher in pollen tube protoplasts. In PT but not in PG protoplasts, inward K+ currents were already detectable at negative membrane voltages usually monitored in lily pollen. $$I_{K^ + in} $$ consisted of a slow and a fast current component, as revealed by fitting a sum of two exponential functions to the time-dependent current. The contribution of the fast component to the total inward current was higher in PT than in PG protoplasts, which was even more evident at acidic pH of the external medium. Therefore, based on the measured characteristics, the $$I_{K^ + in} $$ of PT protoplasts may contribute to the endogenous K+ currents surrounding a growing pollen tube. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Characterization of whole-cell k+ currents across the plasma membrane of pollen grain and tube protoplasts of Lilium longiflorum

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Publisher
Springer-Verlag
Copyright
Copyright © 2003 by Springer-Verlag
Subject
Life Sciences; Biochemistry, general; Human Physiology
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-002-2011-7
Publisher site
See Article on Publisher Site

Abstract

Outward and inward currents, mainly carried by K+, were detected in protoplasts of pollen grains (PG) and pollen tubes (PT) of Lilium longiflorum Thunb. by using the whole-cell configuration of the patch-clamp technique. The outward K+ current $$(I_{K^ + out} )$$ was similar in both protoplast types, while the inward K+ current $$(I_{K^ + in} )$$ was higher in pollen tube protoplasts. In PT but not in PG protoplasts, inward K+ currents were already detectable at negative membrane voltages usually monitored in lily pollen. $$I_{K^ + in} $$ consisted of a slow and a fast current component, as revealed by fitting a sum of two exponential functions to the time-dependent current. The contribution of the fast component to the total inward current was higher in PT than in PG protoplasts, which was even more evident at acidic pH of the external medium. Therefore, based on the measured characteristics, the $$I_{K^ + in} $$ of PT protoplasts may contribute to the endogenous K+ currents surrounding a growing pollen tube.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Mar 25, 2002

References

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