Characterization of unusual G8 rotavirus strains isolated from Egyptian children

Characterization of unusual G8 rotavirus strains isolated from Egyptian children We report the first detection of P(14), G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of the genes encoding VP7 and the VP8 * portion of the VP4 gene. These two strains shared a high level of homology of their VP7s (87.8% nucleotide (nt), 97.2% amino acid (aa)) and VP4s (89.6% nt, 97.1% aa) and had the highest VP7 identity to serotype G8 (>82% nt, >92% aa) and VP4 identity to genotype P(14) (≥81% nt, >91% aa) strains. Serological results with a VP7 G8-specific and VP4 P(14)-specific neutralizing monoclonal antibodies supported the genetic classification of EGY1850 and EGY2295 as P(14), G8. Genogroup analysis supports earlier findings that human G8 rotaviruses may be genetically related to bovine rotaviruses. These findings demonstrate that our understanding of the geographic distribution of rotavirus strains is incomplete, emphasize the need to monitor rota- virus serotypes, and extend the known distribution of serotype G8 and genotype P(14) strains in Africa. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of unusual G8 rotavirus strains isolated from Egyptian children

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Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1999 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050594
Publisher site
See Article on Publisher Site

Abstract

We report the first detection of P(14), G8 rotaviruses isolated in Egypt from the stool of children participating in a 3 year study of rotavirus epidemiology. Two strains, EGY1850 and EGY2295, were characterized by a serotyping enzyme immunoassay (EIA), virus neutralization, and sequence analysis of the genes encoding VP7 and the VP8 * portion of the VP4 gene. These two strains shared a high level of homology of their VP7s (87.8% nucleotide (nt), 97.2% amino acid (aa)) and VP4s (89.6% nt, 97.1% aa) and had the highest VP7 identity to serotype G8 (>82% nt, >92% aa) and VP4 identity to genotype P(14) (≥81% nt, >91% aa) strains. Serological results with a VP7 G8-specific and VP4 P(14)-specific neutralizing monoclonal antibodies supported the genetic classification of EGY1850 and EGY2295 as P(14), G8. Genogroup analysis supports earlier findings that human G8 rotaviruses may be genetically related to bovine rotaviruses. These findings demonstrate that our understanding of the geographic distribution of rotavirus strains is incomplete, emphasize the need to monitor rota- virus serotypes, and extend the known distribution of serotype G8 and genotype P(14) strains in Africa.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 1999

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