Characterization of three anther-specific genes isolated from Chinese cabbage

Characterization of three anther-specific genes isolated from Chinese cabbage Two cDNA libraries were constructed from poly(A)+ RNAs isolated from each of immature flowers (less than 2.0 mm long buds) and anthers (2.0-5.0 mm long buds) of Chinese cabbage (Brassica campestris L. ssp. pekinensis). Using dot-differential hybridization, three cDNA clones, designated BIF38, BAN54, and BAN237, have been isolated from the constructed cDNA libraries and sequenced completely in both directions. Northern blot analyses indicate that all three cDNA clones are abundantly expressed in anther, but not in leaf or other floral organs. The deduced amino acid sequences of BIF38, BAN54, and BAN237 showed high identity with those of known anther-specific genes. Especially the deduced amino acid sequence of BIF38 has 98% identity with that of a phospholipid protein gene (E2) from Brassica napus. Also, the deduced amino acid sequences of BAN54 and BAN237 are similar to the sequences of microspore-specific genes (Bp4A and Bp4C) and pollen oleosins (I3, pol3 and C98), respectively. Southern blot analyses revealed that all three genes belong to multiple gene families in the Chinese cabbage genome. Plant Molecular Biology Springer Journals

Characterization of three anther-specific genes isolated from Chinese cabbage

Loading next page...
Kluwer Academic Publishers
Copyright © 1997 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial