Characterization of the small subgenomic RNA of Soybean dwarf virus

Characterization of the small subgenomic RNA of Soybean dwarf virus We have characterized a small subgenomic RNA of Japanese strains of Soybean dwarf virus (SbDV). Northern blot analyses of SbDV-infected plants showed that the small RNA contained the 3′ terminal sequence of the genome and was detected in four typical Japanese SbDV strains, YS, YP, DS and DP. In the case of SbDV-DS, the RNA was 220 nucleotides in length and was transcribed from the 3′ terminal region of the genome. This RNA appeared at a similar time to genomic RNA and a large sgRNA, and thereafter persisted in the infected plant. Since no conserved open reading frame (ORF) among the strains was postulated in the 3′ terminal region, the small subgenomic RNA may have some regulatory roles in SbDV infections. Archives of Virology Springer Journals

Characterization of the small subgenomic RNA of Soybean dwarf virus

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