Characterization of the nuclear import and export signals of pseudorabies virus UL31

Characterization of the nuclear import and export signals of pseudorabies virus UL31 The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection. Archives of Virology Springer Journals

Characterization of the nuclear import and export signals of pseudorabies virus UL31

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Springer Vienna
Copyright © 2015 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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  • Nucleolar targeting: the hub of the matter
    Emmott, E; Hiscox, JA
  • Nucleocytoplasmic transport
    Gorlich, D; Mattaj, IW

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