Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin

Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin While screening for new antimicrobial agents for multidrug-resistant Salmonella enterica , the novel lytic bacteriophage STP4-a was isolated and characterized. Phage morphology revealed that STP4-a belongs to the family Myoviridae . Bacterial challenge assays showed that different serovars of Salmonella enterica were susceptible to STP4-a infection. The genomic characteristics of STP4-a, containing 159,914 bp of dsDNA with an average GC content of 36.86 %, were determined. Furthermore, the endolysin of STP4-a was expressed and characterized. The novel endolysin, LysSTP4, has hydrolytic activity towards outer-membrane-permeabilized S. enterica and Escherichia coli . These results provide essential information for the development of novel phage-based biocontrol agents against S. enterica . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2647-0
Publisher site
See Article on Publisher Site

Abstract

While screening for new antimicrobial agents for multidrug-resistant Salmonella enterica , the novel lytic bacteriophage STP4-a was isolated and characterized. Phage morphology revealed that STP4-a belongs to the family Myoviridae . Bacterial challenge assays showed that different serovars of Salmonella enterica were susceptible to STP4-a infection. The genomic characteristics of STP4-a, containing 159,914 bp of dsDNA with an average GC content of 36.86 %, were determined. Furthermore, the endolysin of STP4-a was expressed and characterized. The novel endolysin, LysSTP4, has hydrolytic activity towards outer-membrane-permeabilized S. enterica and Escherichia coli . These results provide essential information for the development of novel phage-based biocontrol agents against S. enterica .

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2016

References

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