Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis

Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis A ssRNA virus from kiwifruit ( Actinidia spp. ) was identified as a member of the family Betaflexiviridae . It was mechanically transmitted to the herbaceous indicators Nicotiana benthamiana, N. clevelandii, N. glutinosa and N. occidentalis . The complete genome was comprised of three ORFs and a 3’poly (A) tail. Phylogenetic analysis of the entire genome indicated it was a novel member of the genus Citrivirus (family Betaflexiviridae ). The complete nucleotide sequence differed from that of citrus leaf blotch virus (CLBV) by ~ 26 %. The movement protein (ORF2) and coat protein (ORF3) shared 95-96 % and 90-92 % amino acid sequence identity, respectively, with CLBV. The replicase polyprotein (ORF1) was distinctly different from published CLBV sequences, with 78-79 % amino acid sequence identity, while the 5’ UTR and 3’ UTR differed from CLBV by 28 % and 29 %, respectively. The sequence differences indicate that the citrivirus from Actinidia is either a divergent strain of CLBV or a member of a new citrivirus species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of the complete genome of a novel citrivirus infecting Actinidia chinensis

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1654-2
Publisher site
See Article on Publisher Site

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