Fourteen orthopoxvirus strains isolated from humans, cats, a dog, a cow, and an elephant in Germany were characterized. All were classified as cowpox virus based on haemorrhagic lesions induced on the chorioallantoic membrane of chicken eggs and reactivity of a 160 kDa protein with anti-A-type inclusion protein hyperimmun serum in a Western blot. More detailed comparison of the isolates by restriction endonuclease mapping using Hin dIII and Xho I demonstrated a close relationship between all isolates and confirmed them as cowpox viruses. However, some minor differences between the isolates were detected which proved to be of epidemiological value. One group consisting of five closely related isolates contained a unique 4.0 kb Hin dIII fragment. In a Southern blot this fragment failed to hybridize with other cowpox virus isolates including the reference strain.
Archives of Virology – Springer Journals
Published: Mar 1, 1999
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