Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein

Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and... We have isolated a cDNA encoding a calcium-dependent protein kinase (CDPK) in Nicotiana tabacum, which we designated NtCDPK1. The deduced amino acid sequence of NtCDPK1 suggests that this protein contains the kinase domain at the amino terminus and the autoregulatory and calmodulin-like domains at the carboxy terminus. NtCDPK1 is highly homologous to DcCPK1, a CDPK of carrot, showing 76.5% amino acid sequence identity. NtCDPK1 transcripts are present in roots, stems and flowers, but are almost undetectable in leaves. In leaves, NtCDPK1 mRNA accumulation is stimulated by phytohormones (ABA, GA and cytokinin), Ca2+, methyl jasmonate, wounding, fungal elicitors, chitosan, and NaCl. The recombinant full-length NtCDPK1 protein is catalytically active and highly stimulated by Ca2+. A truncated recombinant NtCDPK1 which lacks the C-terminal calmodulin-homologous domain also undergoes autophosphorylation, but the kinase activity is not stimulated by Ca2+. Phosphoamino acid analysis showed that NtCDPK1 phosphorylates serine and threonine residues. Finally, a 60 kDa protein which matches the expected size of NtCDPK1 was immunodetected in the membrane fraction by an antiserum reacting with NtCDPK1. Immunoprecipitation and in vitro phosphorylation using the antiserum also generated a 60 kDa phosphoprotein only in the membrane fraction. These results suggest that NtCDPK1 is associated with the membrane. Plant Molecular Biology Springer Journals

Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein

Loading next page...
Kluwer Academic Publishers
Copyright © 1999 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
Publisher site
See Article on Publisher Site

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial