Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl-lipid desaturases and thermostable lichenase

Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or... We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2; Δ9,12) accompanied with decrease of α-linolenic acid (C18:3; Δ9,12,15) relative amount was detected for plants expressing hybrid desA::licBM3 gene. No reliable changes were detected in fatty acid profiles and unsaturation index of plants transformed with Δ9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 α-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsat-uration index. No changes in relative amount of Δ9-monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Characterization of Nicotiana tabacum plants expressing hybrid genes of cyanobacterial Δ9 or Δ12 acyl-lipid desaturases and thermostable lichenase

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Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715030073
Publisher site
See Article on Publisher Site

Abstract

We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide of Rubisco small subunit of Arabidopsis thaliana under control of a constitutive promoter. Reliable increase of linoleic acid portion (C18:2; Δ9,12) accompanied with decrease of α-linolenic acid (C18:3; Δ9,12,15) relative amount was detected for plants expressing hybrid desA::licBM3 gene. No reliable changes were detected in fatty acid profiles and unsaturation index of plants transformed with Δ9 desaturase gene desC::licBM3 lacking signals of intracellular targeting while expression of this gene with Arabidopsis thaliana Rubisco small subunit transit peptide sequence caused growth of C18:3 α-linolenic acid part simultaneously with reduction of C18:2 linoleic acid part, as well as increase of unsat-uration index. No changes in relative amount of Δ9-monounsaturated fatty acids were observed in any of studied lines. All plants expressing desaturase genes exhibited enhanced levels of superoxide dismutase (SOD) activity after cold treatment in contrast to control lines with suppressed SOD activity after cold treatment.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Apr 29, 2015

References

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