Characterization of malleability and immunological properties of human adenovirus type 3 hexon hypervariable region 1

Characterization of malleability and immunological properties of human adenovirus type 3 hexon... Adenovirus (Ad) capsids that display exogenous epitopes can be potently immunogenic, eliciting a potent humoral response against components of the capsid. We used the epitopes flag, his 6 flag, his 6 lgsflag and AdV4HVR5 as model antigens to characterize the hexon hypervariable region (HVR) 1 as a site for epitope insertion. A peptide of up to 17 amino acids could be incorporated into HVR1 of the Ad3 hexon without adversely affecting the biological characteristics of the virus. Multiple vaccinations with capsid-modified Ad3 induced a humoral response against the epitope inserted in HVR1. However, antiserum against the his 6 flag or his 6 lgsflag epitope did not recognize glutathione S-transferase (GST)-his 6 and GST-flag fusion protein. Our study illustrates that there is an immune response against the new epitope within the amino acids of his 6 flag or his 6 lgsflag epitopes. This discovery could be a warning for the generation of multivalent vaccine vectors by incorporation of multiple epitopes into single HVRs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of malleability and immunological properties of human adenovirus type 3 hexon hypervariable region 1

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Publisher
Springer Vienna
Copyright
Copyright © 2012 by Springer-Verlag
Subject
Biomedicine; Virology; Infectious Diseases; Medical Microbiology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-012-1364-1
Publisher site
See Article on Publisher Site

Abstract

Adenovirus (Ad) capsids that display exogenous epitopes can be potently immunogenic, eliciting a potent humoral response against components of the capsid. We used the epitopes flag, his 6 flag, his 6 lgsflag and AdV4HVR5 as model antigens to characterize the hexon hypervariable region (HVR) 1 as a site for epitope insertion. A peptide of up to 17 amino acids could be incorporated into HVR1 of the Ad3 hexon without adversely affecting the biological characteristics of the virus. Multiple vaccinations with capsid-modified Ad3 induced a humoral response against the epitope inserted in HVR1. However, antiserum against the his 6 flag or his 6 lgsflag epitope did not recognize glutathione S-transferase (GST)-his 6 and GST-flag fusion protein. Our study illustrates that there is an immune response against the new epitope within the amino acids of his 6 flag or his 6 lgsflag epitopes. This discovery could be a warning for the generation of multivalent vaccine vectors by incorporation of multiple epitopes into single HVRs.

Journal

Archives of VirologySpringer Journals

Published: Sep 1, 2012

References

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