Candidatus Liberibacter asiaticus (Ca. L. asiaticus), a gram negative, phloem-limited, and unculturable bacteria is the causal agent of citrus greening disease. The disease is considered as one of the most serious threats to the citrus industry worldwide. It is responsible for heavy crop losses irrespective of citrus cultivar or rootstock used. During the infection process, the bacterium produces several pathogenesis-related proteins that are crucial for its survival and multiplication in plant phloem tissue and saliva of its insect vectors citrus psylla, inside which it multiplies efficiently. There is a tremendous potential of developing antimicrobial inhibitors against these proteins to reduce pathogen population in plant phloem tissue and in insect vectors as potential tool in disease management strategy. In the present study, we sequenced and characterized four crucial protein genes from 18 samples across India. The four protein genes includes two component response regulator protein, periplasmic solute binding protein, putative protease IV transmembrane protein and serine protease do-like protein of the causal bacterium. Secondary and tertiary structures of the proteins were also predicted using PSIPRED and TMHMM software, respectively. Phylogenetic analyses and secondary protein structure prediction reveals that these four proteins are unique, essential and conserved in Ca. L. asiaticus populations infecting different citrus cultivars prevalent in India. Further, these structures gave an idea about active sites which can act as perfect target sites for blocking. These bacterial encoded proteins are potential targets of antimicrobial inhibitors.
Indian Phytopathology – Springer Journals
Published: May 30, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera