Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination

Characterization of common and distinctive adjustments of wild barley leaf proteome under drought... In nature, plants are often exposed to combinations of different stresses at the same time, while in many laboratory studies of molecular stress induction phenomena, single stress responses are analyzed. This study aims to identify the common (i.e. more general stress-responsive) and the stress-specific adjustments of the leaf proteome of wild barley to two often co-occurring stress phenomena, i.e. in response to (long-term) drought acclimation (DA) or to (transient) heat stress (HS). In addition, we analyzed those alterations which are specific for the combination of both stresses. Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry with a 1.5 threshold value of changes in relative protein contents. DA resulted in specific upregulation of proteins with cell detoxification functions, water homeostasis maintenance, amino acids synthesis and lipid metabolism and distinct forms of heat shock proteins (HSPs) and proteins with chaperon functions while proteins related to nitrogen metabolism were downregulated. This response was distinguished from the response to transient HS, which included upregulation of a broad range of HSP products. The common response to both stressors revealed upregulation of additional forms of HSPs and the downregulation of enzymes of the photosynthetic apparatus and chlorophyll binding proteins. The simultaneous exposure to both stress conditions resulted mostly in a combination of both stress responses and to unique abundance changes of proteins with yet unclear functions. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterization of common and distinctive adjustments of wild barley leaf proteome under drought acclimation, heat stress and their combination

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0291-4
Publisher site
See Article on Publisher Site

Abstract

In nature, plants are often exposed to combinations of different stresses at the same time, while in many laboratory studies of molecular stress induction phenomena, single stress responses are analyzed. This study aims to identify the common (i.e. more general stress-responsive) and the stress-specific adjustments of the leaf proteome of wild barley to two often co-occurring stress phenomena, i.e. in response to (long-term) drought acclimation (DA) or to (transient) heat stress (HS). In addition, we analyzed those alterations which are specific for the combination of both stresses. Leaf proteome analysis was performed using 2D difference gel electrophoresis followed by protein identification via mass spectrometry with a 1.5 threshold value of changes in relative protein contents. DA resulted in specific upregulation of proteins with cell detoxification functions, water homeostasis maintenance, amino acids synthesis and lipid metabolism and distinct forms of heat shock proteins (HSPs) and proteins with chaperon functions while proteins related to nitrogen metabolism were downregulated. This response was distinguished from the response to transient HS, which included upregulation of a broad range of HSP products. The common response to both stressors revealed upregulation of additional forms of HSPs and the downregulation of enzymes of the photosynthetic apparatus and chlorophyll binding proteins. The simultaneous exposure to both stress conditions resulted mostly in a combination of both stress responses and to unique abundance changes of proteins with yet unclear functions.

Journal

Plant Molecular BiologySpringer Journals

Published: Feb 3, 2015

References

  • Chloroplast and mitochondrial proteases in Arabidopsis. A proposed nomenclature
    Adam, Z; Adamska, I; Nakabayashi, K
  • Proteome analysis of soybean roots subjected to short-term drought stress
    Alam, I; Sharmin, SA; Kim, KH; Yang, JK; Choi, MS; Lee, BH
  • Comparative analysis of barley leaf proteome as affected by drought stress
    Ashoub, A; Beckhaus, T; Berberich, T; Karas, M; Brüggemann, W
  • System-based analysis of Arabidopsis leaf growth reveals adaptation to water deficit
    Baerenfaller, K; Massonnet, C; Walsh, S
  • Proteome changes in wild and modern wheat leaves upon drought stress by two-dimensional electrophoresis and nanolc-esi–ms/ms
    Budak, H; Akpinar, BA; Unver, T; Turktas, M
  • Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Activase-mediated activation of Rubisco
    Feller, U; Crafts-Brandner, S; Salvucci, ME

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