Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assay

Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD... Detarium microcarpum is an African tree which is widely used as a folk medicine and food. A methanol extract from the leaves as well as its hexane, dichloromethane, ethyl acetate and n-butanol fractions were evaluated for their total phenolic content and radical scavenging activities. Additionally, a pre-column DPPH-HPLC assay was performed to screen and identify the compounds responsible for the antioxidant properties of the extract. Results indicated that the methanol extract contained a substantial amount of phenolic compounds with a value of 51.7 ± 0.2 mg gallic acid equivalent/g. It also exerted potent radical scavenging activities in both DPPH and ORAC assays, with respective values of 937 ± 16 and 2247 ± 63 μmol trolox equivalent/g. Its ethyl acetate and n-butanol fractions were shown to possess the highest TPC and radical scavenging activities. Pre-column DPPH-HPLC experiments led to the direct detection of five potential radical scavenging compounds which were identified as gallic acid, quercetin 3,7-O-dirhamnoside, myricetin 3-O-rhamnoside, quercetin 3-O-glucoside and quercetin 3-O-rhamnoside. All of them were confirmed to exert potent radical scavenging activities using common DPPH spectrophotometric assay. These findings revealed that D. microcarpum leaves possess potent antioxidant activity and could be recommended as a nutraceutical resource. Additionally, the identified bioactive compounds can be considered as appropriate markers for quality control. European Food Research and Technology Springer Journals

Characterization of antioxidants from Detarium microcarpum Guill. et Perr. leaves using HPLC-DAD coupled with pre-column DPPH assay

Loading next page...
Springer Berlin Heidelberg
Copyright © 2017 by Springer-Verlag Berlin Heidelberg
Chemistry; Food Science; Analytical Chemistry; Biotechnology; Agriculture; Forestry
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial