Characterization of an unusual Ds transposable element in Arabidopsis thaliana: Insertion of an abortive circular transposition intermediate

Characterization of an unusual Ds transposable element in Arabidopsis thaliana: Insertion of an... The maize Ac/Ds transposable elements, which belong to the hAT transposon superfamily, are widely used as insertional mutagens in numerous plant species.Molecular studies suggest that Ac/Ds elements transpose in a conservative non-replicative fashion; however the molecular mechanism of transposition remains unclear. We describe here the identification of an unusual Ds element, Ds-mmd1, in a transgenic Arabidopsis line. Ds-mmd1 is rearranged relative to the original Ds element, such that the original 5′ and 3′ ends are internal and previously internal sequences are the new 5′ and 3′ termini of Ds-mmd1. Short duplications of plant genomic DNA and Ds sequences are present at the Ds-mmd1 junctions, suggesting that a circular Ds molecule was part of the events that created the Ds-mmd1 element. In addition, a revertant analysis on mmd1 plants demonstrated that Ds-mmd1 can be eliminated from the genome in an Ac-dependent process. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterization of an unusual Ds transposable element in Arabidopsis thaliana: Insertion of an abortive circular transposition intermediate

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2004 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-005-2225-z
Publisher site
See Article on Publisher Site

Abstract

The maize Ac/Ds transposable elements, which belong to the hAT transposon superfamily, are widely used as insertional mutagens in numerous plant species.Molecular studies suggest that Ac/Ds elements transpose in a conservative non-replicative fashion; however the molecular mechanism of transposition remains unclear. We describe here the identification of an unusual Ds element, Ds-mmd1, in a transgenic Arabidopsis line. Ds-mmd1 is rearranged relative to the original Ds element, such that the original 5′ and 3′ ends are internal and previously internal sequences are the new 5′ and 3′ termini of Ds-mmd1. Short duplications of plant genomic DNA and Ds sequences are present at the Ds-mmd1 junctions, suggesting that a circular Ds molecule was part of the events that created the Ds-mmd1 element. In addition, a revertant analysis on mmd1 plants demonstrated that Ds-mmd1 can be eliminated from the genome in an Ac-dependent process.

Journal

Plant Molecular BiologySpringer Journals

Published: Jan 26, 2005

References

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