Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris

Characterization of an abundant short interspersed nuclear element (SINE) present in Canis... A short interspersed nuclear element (Can SINE) of ∼130–150 bp was cloned and characterized from Canis familiaris. We demonstrate that this element is interspersed, present approximately every 5–8.3 kbp, and many are sufficiently close to allow IRS (interspersed repetitive DNA sequences) PCR. Sequence analysis of > 20 Can SINEs from the dog has identified a conserved region that was used to design oligonucleotides for IRS PCR. Since Can SINEs are not present in human or rodent genomes, IRS PCR using oligonucleotides directed to the conserved region of Can SINEs can be used to simplify analysis of canid DNA in somatic cell hybrids, as well as in large insert cloning vectors. We demonstrate that the canid IRS products are polymorphic and could be developed as genetic markers for filter-based genotyping in this organism. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris

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Publisher
Springer-Verlag
Copyright
Copyright © 1998 by Springer-Verlag New York Inc
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
D.O.I.
10.1007/s003359900681
Publisher site
See Article on Publisher Site

Abstract

A short interspersed nuclear element (Can SINE) of ∼130–150 bp was cloned and characterized from Canis familiaris. We demonstrate that this element is interspersed, present approximately every 5–8.3 kbp, and many are sufficiently close to allow IRS (interspersed repetitive DNA sequences) PCR. Sequence analysis of > 20 Can SINEs from the dog has identified a conserved region that was used to design oligonucleotides for IRS PCR. Since Can SINEs are not present in human or rodent genomes, IRS PCR using oligonucleotides directed to the conserved region of Can SINEs can be used to simplify analysis of canid DNA in somatic cell hybrids, as well as in large insert cloning vectors. We demonstrate that the canid IRS products are polymorphic and could be developed as genetic markers for filter-based genotyping in this organism.

Journal

Mammalian GenomeSpringer Journals

Published: Mar 28, 2009

References

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