Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata

Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance... Vacuolar processing enzymes are cysteine proteases responsible for maturation of vacuolar proteins. They have been shown to possess caspase-1-like activity, mediate cell death and display increased activity during pathogen infections. A transcript derived fragment corresponding to VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, Arachis diogoi upon challenge from the late leaf spot pathogen Phaeoisariopsis personata, which was subsequently validated by q-PCR in a time course analysis, where susceptible peanut did not show its upregulation. In transient conditional and constitutive expression studies in tobacco leaves using agroinfiltration, we have observed that expression of AdVPE was associated with hypersensitive response (HR) like cell death. AdVPE expression was found to be high at 24 h post estradiol application and this was associated with the enhanced co-expression of molecular markers of HR cell death genes and genes for pathogenesis related proteins indicating that AdVPE positively regulates defense responses and its estradiol induced expression is sufficient for HR-like cell death in tobacco. We found that AdVPE expression was very strongly induced in response to sodium nitroprusside, which indicates its involvement in stress signaling. Induced expression of AdVPE in response to jasmonic acid and ethylene also indicates its involvement in an interconnected network of signaling. Transgenic tobacco plants ectopically expressing AdVPE exhibited enhanced resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var.  nicotianae and Rhizoctonia solani. To our knowledge, this is the first report on the heterologous expression of a pathogen induced VPE enhancing resistance to fungal pathogens with cell death phenomenon under transient expression. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterization of a vacuolar processing enzyme expressed in Arachis diogoi in resistance responses against late leaf spot pathogen, Phaeoisariopsis personata

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0318-x
Publisher site
See Article on Publisher Site

Abstract

Vacuolar processing enzymes are cysteine proteases responsible for maturation of vacuolar proteins. They have been shown to possess caspase-1-like activity, mediate cell death and display increased activity during pathogen infections. A transcript derived fragment corresponding to VPE was found to be up-regulated in a cDNA-AFLP analysis of host responses of a wild peanut, Arachis diogoi upon challenge from the late leaf spot pathogen Phaeoisariopsis personata, which was subsequently validated by q-PCR in a time course analysis, where susceptible peanut did not show its upregulation. In transient conditional and constitutive expression studies in tobacco leaves using agroinfiltration, we have observed that expression of AdVPE was associated with hypersensitive response (HR) like cell death. AdVPE expression was found to be high at 24 h post estradiol application and this was associated with the enhanced co-expression of molecular markers of HR cell death genes and genes for pathogenesis related proteins indicating that AdVPE positively regulates defense responses and its estradiol induced expression is sufficient for HR-like cell death in tobacco. We found that AdVPE expression was very strongly induced in response to sodium nitroprusside, which indicates its involvement in stress signaling. Induced expression of AdVPE in response to jasmonic acid and ethylene also indicates its involvement in an interconnected network of signaling. Transgenic tobacco plants ectopically expressing AdVPE exhibited enhanced resistance against Phytophthora parasitica var. nicotianae, Alternaria alternata var.  nicotianae and Rhizoctonia solani. To our knowledge, this is the first report on the heterologous expression of a pathogen induced VPE enhancing resistance to fungal pathogens with cell death phenomenon under transient expression.

Journal

Plant Molecular BiologySpringer Journals

Published: Apr 17, 2015

References

  • The genetics and pathology of Rhizoctonia solani
    Anderson, NA
  • ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis
    Bright, J; Desikan, R; Hancock, JT; Weir, IS; Neill, SJ
  • Biosynthesis and action of jasmonates in plants
    Creelman, RA; Mullet, JE
  • Redundant proteolytic mechanisms process seed storage proteins in the absence of seed-type members of the vacuolar processing enzyme family of cysteine proteases
    Gruis, DF; Selinger, DA; Curran, JM; Jung, R
  • Proglobulin processing enzyme in vacuoles isolated from developing pumpkin cotyledons
    Hara-Nishimura, I; Nishimura, M
  • Biosynthesis and intracellular transport of 11S globulin in developing pumpkin cotyledons
    Hara-Nishimura, I; Nishimura, M; Akazawa, T
  • A cellular suicide strategy of plants: vacuole-mediated cell death
    Hatsugai, N; Kuroyanagi, M; Nishimura, M; Hara-Nishimura, I
  • Expression and activation of the vacuolar processing enzyme in Saccharomyces cerevisiae
    Hiraiwa, N; Nishimura, M; Hara-Nishimura, I
  • Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions
    Kinoshita, T; Yamada, K; Hiraiwa, N; Kondo, M; Nishimura, M; Hara-Nishimura, I
  • Differential gene expression in Arachis diogoi upon interaction with peanut late leaf spot pathogen Phaeoisariopsis personata and characterization of a pathogen induced cyclophilin
    Kumar, KR; Kirti, PB
  • Novel role for a serine/arginine-rich splicing factor, AdRSZ21 in plant defense and HR-like cell death
    Kumar, KR; Kirti, PB

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