Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus

Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship... Xanthophylls are oxygenated carotenoids that perform critical roles in plants. β-carotene hydroxylases (β-hydroxylases) add hydroxyl groups to the β-rings of carotenes and have been cloned from several bacteria and plants, including Arabidopsis. The lut1 mutation of Arabidopsis disrupts ε-ring hydroxylation and has been suggested to identify a related carotene hydroxylase that functions specifically on ε-ring structures. We have used library screening and genomics-based approaches to isolate a second β-hydroxylase genomic clone and its corresponding cDNA from Arabidopsis. The encoded protein is 70% identical to the previously reported Arabidopsis β-hydroxylase 1. Phylogenetic analysis indicates a common origin for the two proteins, however, their different chromosomal locations, intron positions and intron sizes suggest their duplication is not recent. Although both hydroxylases are expressed in all Arabidopsis tissues analyzed, β-hydroxylase 1 mRNA is always present at higher levels. Both cDNAs encode proteins that efficiently hydroxylate the C-3 position of β-ring containing carotenes and are only weakly active towards ε-ring containing carotenes. Neither β-hydroxylase cDNA maps to the LUT1 locus, and the genomic region encompassing the LUT1 locus does not contain a third related hydroxylase. These data indicate that the LUT1 locus encodes a protein necessary for ε-ring hydroxylation but unrelated to β-hydroxylases at the level of amino acid sequence. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterization of a second carotenoid β-hydroxylase gene from Arabidopsis and its relationship to the LUT1 locus

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 2001 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1011623907959
Publisher site
See Article on Publisher Site

Abstract

Xanthophylls are oxygenated carotenoids that perform critical roles in plants. β-carotene hydroxylases (β-hydroxylases) add hydroxyl groups to the β-rings of carotenes and have been cloned from several bacteria and plants, including Arabidopsis. The lut1 mutation of Arabidopsis disrupts ε-ring hydroxylation and has been suggested to identify a related carotene hydroxylase that functions specifically on ε-ring structures. We have used library screening and genomics-based approaches to isolate a second β-hydroxylase genomic clone and its corresponding cDNA from Arabidopsis. The encoded protein is 70% identical to the previously reported Arabidopsis β-hydroxylase 1. Phylogenetic analysis indicates a common origin for the two proteins, however, their different chromosomal locations, intron positions and intron sizes suggest their duplication is not recent. Although both hydroxylases are expressed in all Arabidopsis tissues analyzed, β-hydroxylase 1 mRNA is always present at higher levels. Both cDNAs encode proteins that efficiently hydroxylate the C-3 position of β-ring containing carotenes and are only weakly active towards ε-ring containing carotenes. Neither β-hydroxylase cDNA maps to the LUT1 locus, and the genomic region encompassing the LUT1 locus does not contain a third related hydroxylase. These data indicate that the LUT1 locus encodes a protein necessary for ε-ring hydroxylation but unrelated to β-hydroxylases at the level of amino acid sequence.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 3, 2004

References

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