Characterization of a potyvirus isolated from Tradescantia fluminensis in northern Italy

Characterization of a potyvirus isolated from Tradescantia fluminensis in northern Italy We isolated a potyvirus from Tradescantia fluminensis that was causing leaf distortion and mild mosaic. We cloned and sequenced a 1500 bp cDNA obtained by RT-PCR corresponding to the 3′ proximal region of the genome. We determined the host range and tested a series of potyviral antisera against our tradescantia virus isolate by immuno-enzymatic methods. Based on our results, we suggest that our viral isolate could be considered a new potyvirus species named Tradescantia mild mosaic potyvirus. Phylogenetic analysis confirmed that Tradescantia mild mosaic virus belongs to the genus Potyvirus within the family Potyviridae , but the virus could not be assigned to any of the potyvirus groupings recently defined. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of a potyvirus isolated from Tradescantia fluminensis in northern Italy

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Publisher
Springer-Verlag
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Biomedicine; Medical Microbiology; Infectious Diseases; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-005-0691-x
Publisher site
See Article on Publisher Site

Abstract

We isolated a potyvirus from Tradescantia fluminensis that was causing leaf distortion and mild mosaic. We cloned and sequenced a 1500 bp cDNA obtained by RT-PCR corresponding to the 3′ proximal region of the genome. We determined the host range and tested a series of potyviral antisera against our tradescantia virus isolate by immuno-enzymatic methods. Based on our results, we suggest that our viral isolate could be considered a new potyvirus species named Tradescantia mild mosaic potyvirus. Phylogenetic analysis confirmed that Tradescantia mild mosaic virus belongs to the genus Potyvirus within the family Potyviridae , but the virus could not be assigned to any of the potyvirus groupings recently defined.

Journal

Archives of VirologySpringer Journals

Published: Jun 1, 2006

References

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