Characterization of a novel negevirus isolated from Aedes larvae collected in a subarctic region of Japan

Characterization of a novel negevirus isolated from Aedes larvae collected in a subarctic region... We isolated and characterized a novel positive-sense, single-stranded RNA virus from Aedes larvae collected on Okushiri Island, Hokkaido, Japan. This virus, designated Okushiri virus (OKV), replicated in the Aedes albopictus cell line C6/36 with severe cytopathic effects and produced a large number of spherical viral particles that were 50-70 nm in diameter and released into the cell culture medium. The OKV genome consisted of 9,704 nucleotides, excluding the poly(A) tail at the 3′-terminus, and contained three major open reading frames (ORF1, ORF2, and ORF3). ORF1 encoded a putative protein of approximately 268 kDa that included a methyltransferase domain, FtsJ-like methyltransferase domain, helicase domain, and RNA-dependent RNA polymerase domain. The genome organization and results of a phylogenetic analysis based on the amino acid sequence predicted from the nucleotide sequence indicated that OKV is a member of a new insect virus group of negeviruses with a possible evolutionary relationship to some plant viruses. ORF2 and ORF3 were suggested to encode hypothetical membrane-associated proteins of approximately 45 kDa and 22 kDa, respectively. This is the first study on a novel negevirus isolated from mosquito larvae in Japan. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of a novel negevirus isolated from Aedes larvae collected in a subarctic region of Japan

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2711-9
Publisher site
See Article on Publisher Site

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    Adams, MJ; Lefkowitz, EJ; King, AMQ; Carstens, EB
  • Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2009)
    Carstens, EB
  • Development of a strand-specific RT-PCR based assay to detect the replicative form of hepatitis C virus RNA
    Craggs, JK; Ball, JK; Thomson, BJ; Irving, WL; Grabowska, AM
  • SOSUI: classification and secondary structure prediction system for membrane proteins
    Hirokawa, T; Boon-Chieng, S; Mitaku, S

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