Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus

Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii . In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the genomic DNA of DkNPV using a DNA fragment library. The DkChi gene, encoding 558 residues protein with a predicted mass of 61.6 kDa, was expressed at high levels in Escherichia coli and purified by affinity chromatography. We confirmed that the prepared protein was the DkChi protein by mass spectrometry analysis. Enzyme activity analysis showed that DkChi had both endo- and exo-chitinase activities. Interestingly, the DkChi protein displayed a strong insecticidal activity against Spodoptera exigua , Hyphantria cunea , Helicoverpa armigera and Lymantria dispar . The results suggest that DkChi is a good candidate protein for significantly contributing to pest control. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus

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Publisher
Springer Vienna
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1775-7
Publisher site
See Article on Publisher Site

Abstract

Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii . In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the genomic DNA of DkNPV using a DNA fragment library. The DkChi gene, encoding 558 residues protein with a predicted mass of 61.6 kDa, was expressed at high levels in Escherichia coli and purified by affinity chromatography. We confirmed that the prepared protein was the DkChi protein by mass spectrometry analysis. Enzyme activity analysis showed that DkChi had both endo- and exo-chitinase activities. Interestingly, the DkChi protein displayed a strong insecticidal activity against Spodoptera exigua , Hyphantria cunea , Helicoverpa armigera and Lymantria dispar . The results suggest that DkChi is a good candidate protein for significantly contributing to pest control.

Journal

Archives of VirologySpringer Journals

Published: Dec 1, 2013

References

  • The molecular biology of chitin digestion
    Cohen-Kupiec, R; Chet, I
  • N -Acetylglucosaminidase (chitobiase) from Serratia marcescens : gene sequence, and protein production and purification in Escherichia coli
    Tews, I; Vincentelli, R; Vorgias, CE
  • Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
    Wang, H; Wu, D; Deng, F; Peng, H; Chen, X; Lauzon, H; Arif, BM; Jehle, JA; Hu, Z
  • Influences of chitinase gene deletion from BmNPV on the cell lysis and host liquefaction
    Wang, F; Zhang, C; Shyam Kumar, V; Wu, X

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