Plant Molecular Biology (2018) 97:279–296 https://doi.org/10.1007/s11103-018-0741-x Characterization of 25 full-length S-RNase alleles, including flanking regions, from a pool of resequenced apple cultivars 1 2 2 1 2,3 Paolo De Franceschi · Luca Bianco · Alessandro Cestaro · Luca Dondini · Riccardo Velasco Received: 22 January 2018 / Accepted: 17 May 2018 / Published online: 29 May 2018 © Springer Science+Business Media B.V., part of Springer Nature 2018 Key message Data obtained from Illumina resequencing of 63 apple cultivars were used to obtain full-length S-RNase sequences using a strategy based on both alignment and de novo assembly of reads. Abstract The reproductive biology of apple is regulated by the S-RNase-based gametophytic self-incompatibility system, that is genetically controlled by the single, multi-genic and multi-allelic S locus. Resequencing of apple cultivars provided a huge amount of genetic data, that can be aligned to the reference genome in order to characterize variation to a genome-wide level. However, this approach is not immediately adaptable to the S-locus, due to some peculiar features such as the high degree of polymorphism, lack of colinearity between haplotypes and extensive presence of repetitive elements. In this study we describe a dedicated procedure aimed at characterizing S-RNase alleles from resequenced cultivars. The S-genotype of 63 apple accessions is reported; the
Plant Molecular Biology – Springer Journals
Published: May 29, 2018
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