Arch Virol (2006) 151: 1179–1188
Characterization and partial genome sequence of stocky
prune virus, a new member of the genus Cheravirus
T. Candresse, L. Svanella-Dumas, and O. Le Gall
Equipe de Virologie, UMR GDPP, INRA et Universit´e Bordeaux 2,
IBVM, Campus INRA, Villenave d’Ornon, France
Received August 4, 2005; accepted October 18, 2005
Published online December 29, 2005
Summary. Characterization of a seemingly new spherical virus isolated from
severely affected plum trees in south-western France indicated that its divided
genome is composed of two single-stranded, polyadenylated RNAs of approxi-
mately 7.4 and 3.7 kb. Its particles are composed of three coat protein subunits of
approximately 23, 23.5, and 24.5 kDa. Partial sequencing of the genomic RNAs
indicated that this new virus, tentatively named stocky prune virus (StPV), is
distantly related to the two sequenced cheraviruses, cherry rasp leaf virus (CRLV)
and apple latent spherical virus (ALSV). StPV should be regarded as a new
member in the unassigned genus Cheravirus.
The newly recognized genus Cheravirus [21, 24] groups a phylogenetically coher-
ent set of plant pathogenic viruses previously recognized as atypical nepoviruses.
The genus Cheravirus is unassigned but has relationships with the family
Sequiviridae . In particular, cheraviruses differ from nepoviruses in having
three small (ca. 20–25 kDa) coat protein subunits. So far, sequence information
is only available for two members of the genus, the type species Cherry rasp
leaf virus (CRLV) [13, 14, 28] and Apple latent spherical virus (ALSV) .
Several other viruses have been described as having three small coat protein
subunits, such as artichoke vein banding virus (AVBV) [7, 8] or Arracacha virus
B (AVB) [16, 18] and are tentatively included in the genus Cheravirus [21, 24].
Some years ago, a seemingly new spherical virus was identiﬁed after mechanical
inoculation of Chenopodium murale plants with extracts from “stocky prune
The nucleotide sequences reported in this manuscript have been deposited in Genbank
under the Accession numbers DQ143874 (RNA1) and DQ143875 (RNA2).