Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes

Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus... A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae , and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3–11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1667-x
Publisher site
See Article on Publisher Site

Abstract

A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae , and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3–11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes .

Journal

Archives of VirologySpringer Journals

Published: Aug 1, 2013

References

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