Characterization and expression of a rice RAD23 gene

Characterization and expression of a rice RAD23 gene In order to identify proteins that interact with plant transcriptional complexes, we performed a two-hybrid screen in yeast using a cDNA library from embryogenic rice suspension cultures and the plant transcriptional activator viviparous-1 (vp1) as ‘bait’. In this screen, we detected an interaction between VP1 and a rice homologue of the Saccharomyces cerevisiae RAD23 gene (osRAD23). The RAD23 protein is associated with the general transcriptional machinery in yeast, and is believed to play a role in the processes of nucleotide excision repair in yeast and mammalian cells. This report is the first identification of a RAD23 homolog in plants. The osRAD23 amino acid sequence shares 50–60% similarity throughout its length with RAD23 sequences from yeast, mice, and man. osRAD23 contains a characteristic ubiquitin-like domain at its N-terminus, which is similar to other RAD23 genes. Analysis of the expressed sequence tag database identifies two different classes of RAD23 genes in both Arabidopsis and rice. Southern analysis of rice genomic DNA indicated the presence of at least two RAD23-like genes. A single transcript (1.5 kb) of osRAD23 was detected in total RNA from rice embryonic tissue, while three transcripts (1.8, 1.5 and 1.0 kb) were observed in total RNA from vegetative tissues of rice. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterization and expression of a rice RAD23 gene

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Publisher
Kluwer Academic Publishers
Copyright
Copyright © 1997 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005870227632
Publisher site
See Article on Publisher Site

Abstract

In order to identify proteins that interact with plant transcriptional complexes, we performed a two-hybrid screen in yeast using a cDNA library from embryogenic rice suspension cultures and the plant transcriptional activator viviparous-1 (vp1) as ‘bait’. In this screen, we detected an interaction between VP1 and a rice homologue of the Saccharomyces cerevisiae RAD23 gene (osRAD23). The RAD23 protein is associated with the general transcriptional machinery in yeast, and is believed to play a role in the processes of nucleotide excision repair in yeast and mammalian cells. This report is the first identification of a RAD23 homolog in plants. The osRAD23 amino acid sequence shares 50–60% similarity throughout its length with RAD23 sequences from yeast, mice, and man. osRAD23 contains a characteristic ubiquitin-like domain at its N-terminus, which is similar to other RAD23 genes. Analysis of the expressed sequence tag database identifies two different classes of RAD23 genes in both Arabidopsis and rice. Southern analysis of rice genomic DNA indicated the presence of at least two RAD23-like genes. A single transcript (1.5 kb) of osRAD23 was detected in total RNA from rice embryonic tissue, while three transcripts (1.8, 1.5 and 1.0 kb) were observed in total RNA from vegetative tissues of rice.

Journal

Plant Molecular BiologySpringer Journals

Published: Sep 29, 2004

References

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