1022-7954/01/3705- $25.00 © 2001
Russian Journal of Genetics, Vol. 37, No. 5, 2001, pp. 513–518. Translated from Genetika, Vol. 37, No. 5, 2001, pp. 631–637.
Original Russian Text Copyright © 2001 by Yermolaev, Savina, Gorelov, Knyazev, Mitichashvili, Mirtskhoulava.
The purpose of this study was to ﬁnd the possible
genealogical relationships between pig breeds studied
and other populations of hogs.
MATERIALS AND METHODS
We studied the following pig breeds: Large White
(samples from Georgian farms and the Bol’shevik Stock-
breeding Farm, Novosibirsk oblast), Lithuanian White,
Swedish Landrace, Kakhetinskaya, Svanetskaya (Geor-
gian farms), Kemerovskaya (the Chkalovskii and Yur-
ginskii stockbreeding farms, Kemerovo oblast), and
Northern Siberian (Cherepanovskoe Stockbreeding Cor-
poration, Novosibirsk oblast).
Blood serum of different wild boar subspecies was
sampled during scheduled trappings and shootings.
Samples of blood serum of the European boar subspe-
Sus scrofa scrofa
L.) were collected in the
Belovezhskaya Pushcha, Berezinskii, and Voronezhskii
state reserves. Samples of blood serum of the Cauca-
sian wild boar subspecies (
S. s. attila
) were obtained
from Transcaucasian (Azerbaijani and Adygei) popula-
tions. Blood of the Siberian subspecies (
S. s. sibiricus
was mainly collected from boars inhabiting the
Bol’shoe Khapto and Maloe Khapto hunting grounds
(Kaa-Khem raion, Tuva Republic). Other samples were
obtained from boars from the Tapsy hunting ground
(Kyzyl raion) and Tes-Khem raion (Tuva Republic).
For typing, we used the bank of reagents for hog
serum allotypes obtained at the Institute of Cytology
and Genetics (Siberian Division, Russian Academy of
Sciences) , all of which passed the international
comparison test in 1987–1988. All animals were typed
for ﬁve allotypes of serum
-macroglobulins, two allo-
types of immunoglobulins G, allotypes of high- and
low-density lipoproteins, and allotypes of some serum
immunogenetic systems identiﬁed less accurately.
The methods used for double immunodiffusion and
immunoelectrophoresis were tested earlier . The
haplotypic and phenotypic frequencies were calculated
as described in . Genetic similarity and genetic dis-
tances between populations were estimated using
UPGMA [4, 5].
The results obtained are shown in the table and, as a
dendrogram, in the ﬁgure. We used the following allotypic
systems to estimate the immunogenetic polymorphism of
the aforementioned pig breeds and wild boar subspecies.
The frequencies of the allotype G1 in different
breeds of pigs considerably differed from one another;
the maximum difference was sixfold (table). Among
domesticated pigs, the Northern Siberian breed exhib-
ited the highest G1 frequency (0.29); it was the same as
in European wild boars. The lowest G1 frequencies
were observed in Svanetskaya (0.05) and Kakhetin-
skaya pigs (0.08) from Georgia. The maximum possi-
ble G1 frequency (1.00) was found in Siberian wild
boars. Note that the breeds with contrasting G1 frequen-
cies are shown close to each other in the dendrogram.
The allotype G4 was absent in small proportions of
Kemerovskaya and Northern Siberian pigs. In other
Characteristics of Immunogenetic Systems
and Blood Serum Proteins in Some Pig Breeds and Georgian
and Western Siberian Wild Boar Populations
V. I. Yermolaev
, M. A. Savina
, I. G. Gorelov
, S. P. Knyazev
R. S. Mitichashvili
, and E. G. Mirtskhoulava
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, 630090 Russia;
fax: (3832) 33-12-78
Novosibirsk State Agrarian University, Department of Animal Breeding and Genetics, Department of Pig Breeding,
Novosibirsk, 630039 Russia
Received August 21, 2000
—A bank of reagents for hog serum protein allotypes has been created. All of these allotypes passed
international comparison tests in 1987–1988. The bank can be used for typing the animals for four generally
accepted (Gp, LpB, Lpr, and IgGH) and several experimental systems. In this study, immunogenetic characteris-
tics of some pig breeds (Large White, Lithuanian White, Swedish Landrace, Kakhetinskaya, and Svanetskaya)
bred in Georgia are compared with those of western Siberian breeds (Large White, Kemerovskaya, and Northern
Siberian), and some foreign breeds, as well as with European, Caucasian, and Siberian subspecies of the wild boar.