Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group protein HMG-I/Y

Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group... The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence contains a short open reading frame of 13 amino acid residues. The 5′-upstream region of 2117 bp and several 5′ deletions were fused to the β-glucuronidase (GUS) reporter gene and transferred to tobacco by Agrobacterium-mediated transformation. Analysis of transgenic tobacco plants containing HMG-I/Y promoter regions of -2117, -1468 and -707 from the translation start detected GUS activity in all organs examined, including roots, stems, leaves and floral organs. Deletion from -707 to -185 resulted in a 20–30-fold reduction in GUS activity in roots and stems, indicating the presence of important quantitative regulatory elements in this region. Plant Molecular Biology Springer Journals

Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group protein HMG-I/Y

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Kluwer Academic Publishers
Copyright © 1998 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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