Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group protein HMG-I/Y

Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group... The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence contains a short open reading frame of 13 amino acid residues. The 5′-upstream region of 2117 bp and several 5′ deletions were fused to the β-glucuronidase (GUS) reporter gene and transferred to tobacco by Agrobacterium-mediated transformation. Analysis of transgenic tobacco plants containing HMG-I/Y promoter regions of -2117, -1468 and -707 from the translation start detected GUS activity in all organs examined, including roots, stems, leaves and floral organs. Deletion from -707 to -185 resulted in a 20–30-fold reduction in GUS activity in roots and stems, indicating the presence of important quantitative regulatory elements in this region. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group protein HMG-I/Y

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Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1023/A:1005928219895
Publisher site
See Article on Publisher Site

Abstract

The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence contains a short open reading frame of 13 amino acid residues. The 5′-upstream region of 2117 bp and several 5′ deletions were fused to the β-glucuronidase (GUS) reporter gene and transferred to tobacco by Agrobacterium-mediated transformation. Analysis of transgenic tobacco plants containing HMG-I/Y promoter regions of -2117, -1468 and -707 from the translation start detected GUS activity in all organs examined, including roots, stems, leaves and floral organs. Deletion from -707 to -185 resulted in a 20–30-fold reduction in GUS activity in roots and stems, indicating the presence of important quantitative regulatory elements in this region.

Journal

Plant Molecular BiologySpringer Journals

Published: Oct 6, 2004

References

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