The interaction between auxin and oligosaccharin OS-RG of natural origin (DP ∼10) during the process of adventitious root formation was studied. Oligosaccharin isolated from pea seedlings increased by 20–25% the number of roots induced by IAA on both the segments of buckwheat (Fagopyrum esculentum Moench) hypocotyls and explants produced from the leaves of transgenic (rolB-GUS) tobacco Nicotiana tabacum L., cv Petit Havana. The highest effect was obtained after short-term treatment of explants with oligosaccharin before hormone adding. The optimal time of pretreatment depended on the used model system and was from 1–2 to 5–24 h for buckwheat hypocotyl segments and explants from tobacco leaves, respectively. Treatment with OS-RG after IAA did not affect the number of roots induced by the hormone. By using the explants from the leaves of transgenic tobacco plants harboring the reporter GUS gene under the control of the auxin-inducible promoter of the rolB gene permitted to reveal the explant response to the hormone at the early stages of root formation. The dynamics of GUS activity after IAA addition was characterized by the presence of two peaks. The histological analysis showed that the first peak coincided with the formation of 4–5-layer primordia, whereas the second peak — with the emergence of essentially developed roots. Pretreatment of explants from tobacco leaves with OS-RG activated IAA-induced GUS-activity and accelerated the response, i.e., the shift of the first peak to the beginning of culture without change in the position of the second peak. Thus, obtained data indicate that OS-RG action precedes that of the hormone at early stages of rhizogenesis. Possible mechanisms of interaction between IAA and oligosaccharin in the process of root formation are discussed.
Russian Journal of Plant Physiology – Springer Journals
Published: Feb 15, 2015
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