1021-4437/04/5104- © 2004
Russian Journal of Plant Physiology, Vol. 51, No. 4, 2004, pp. 500–507. From Fiziologiya Rastenii, Vol. 51, No. 4, 2004, pp. 555–562.
Original English Text Copyright © 2004 by Abrishamchi, Radjabian, Ebrahimzadeh.
It is usually believed that the transition of a shoot
meristem from the vegetative to the ﬂowering state
requires the expression of new genetic information .
During ﬂowering, some genes inactive in the earlier
vegetative period are expressed. The expression of
these genes should be accompanied by the occurrence
of speciﬁc proteins of ﬂowering which were absent
from vegetative plant [2, 3]. Since the 1960s, much
interest has been focused on the time when such
changes in the pattern of gene expression (and/or the
resulting changes in the proteins) can ﬁrst be detected
in the apical meristems. Several attempts to resolve this
problem have been reported with
(saffron), a perennial and herbaceous
plant belonging to the Iridaceae family, is sexually ster-
ile  and propagated vegetatively by the corms .
Saffron plant does not require the light regime for the
induction of ﬂowering. Signiﬁcant ﬂoral development
is supported exclusively by reserve materials stored in
the corm tissues . Some aspects of growth, includ-
ing ﬂower differentiation in saffron plant, have been
studied [12, 13]. In continuation of the earlier investi-
Changes in the Polypeptide Composition during the Ontogenetic
Development of the Shoot Apex of
P. Abrishamchi*, T. Radjabian**, and H. Ebrahimzadeh***
*Department of Biology, Faculty of Science, Ferdowsi University, Mashad, Iran;
e-mail: firstname.lastname@example.org; email@example.com
**Department of Biology, Faculty of Science, Shahed University, Tehran, Iran
***Department of Biology, Faculty of Science, Tehran University, Tehran, Iran
Received January 14, 2003
—The shoot apex development during the life cycle of
L. was characterized by light
microscopy and two-dimensional gel electrophoresis. Using silver staining of polyacrylamide gels, numerous
quantitative and qualitative changes in the populations of polypeptides were observed during transition from
vegetative to preﬂoral and from preﬂoral to ﬂoral stages. Using 80
g protein, we were able to detect 352
polypeptidic spots. In comparison with the vegetative apex, 89 new polypeptides were identiﬁed in the preﬂoral
meristem and 29 polypeptides were missing. In the reproductive meristem, 94 new spots were identiﬁed and 44
spots were missing. Thus, substantial quantitative and qualitative changes in the populations of polypeptides
occurred during the preﬂoral stage, a point of no return in plant development, i.e., and before ﬂoral primordia
Key words: Crocus sativus - preﬂoral meristem - reproductive meristem - shoot apex - two-dimensional gel
: DTT—dithiothreitol; FAA—formalin–acetic
acid–alcohol; PVPP—polyvinylpolypyrrolidone; 2D-PAGE—
two-dimensional polyacrylamide gel electrophoresis.
This article was submitted by the authors in English.
Vegetative meristem at the early stage of formation
with 2–3 layered tunica (t) and a small corpus (c).
(1) Apical zone, (2) lateral zone, and (3) rib meristem are
distinguished. Anticlinal divisions of tunica layers (single
arrow) and periclinal divisions of subtunica layers (double
arrow) in lateral meristem produce a primordium of a cov-
ering scale (