Changes in the Contents of Inactive Complexes of Photosystem II in Chlorella Cells Incubated in the Light and Darkness

Changes in the Contents of Inactive Complexes of Photosystem II in Chlorella Cells Incubated in... A prolonged (20–24 h) dark incubation of Chlorella pyrenoidosa algae at 37–38°С did not diminish the relative yield of the variable chlorophyll fluorescence (F v/F m) and enhanced the relative contribution of the slow phase (sF v) to the kinetics of F v increase. Iodoacetamide, a nonmetabolized glucose analog, 2-deoxyglucose (2-DG), an inhibitor of protein synthesis, cycloheximide, and a decrease in the temperature of dark incubation to 18–20°С prevented this sF v increase. Both the illumination of dark-incubated cells and the addition of 2-DG in darkness restored the initial level of sF v. In the light-grown chlorella cells, the relative contribution of sF v reversibly declined with lowering light intensity and increased when СО2 was excluded from the bubbling mixture. The authors presume that the slow phase in the kinetics of F v increase is related to the functioning of the fraction of the photosystem II complexes with a destabilized primary quinone acceptor of electron, and the content of these complexes in the cell depends on the plastoquinone redox state. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Changes in the Contents of Inactive Complexes of Photosystem II in Chlorella Cells Incubated in the Light and Darkness

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Publisher
Springer Journals
Copyright
Copyright © 2004 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/B:RUPP.0000028673.79956.a2
Publisher site
See Article on Publisher Site

Abstract

A prolonged (20–24 h) dark incubation of Chlorella pyrenoidosa algae at 37–38°С did not diminish the relative yield of the variable chlorophyll fluorescence (F v/F m) and enhanced the relative contribution of the slow phase (sF v) to the kinetics of F v increase. Iodoacetamide, a nonmetabolized glucose analog, 2-deoxyglucose (2-DG), an inhibitor of protein synthesis, cycloheximide, and a decrease in the temperature of dark incubation to 18–20°С prevented this sF v increase. Both the illumination of dark-incubated cells and the addition of 2-DG in darkness restored the initial level of sF v. In the light-grown chlorella cells, the relative contribution of sF v reversibly declined with lowering light intensity and increased when СО2 was excluded from the bubbling mixture. The authors presume that the slow phase in the kinetics of F v increase is related to the functioning of the fraction of the photosystem II complexes with a destabilized primary quinone acceptor of electron, and the content of these complexes in the cell depends on the plastoquinone redox state.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 18, 2004

References

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