Latent period, burst time, and burst size, kinetic parameters of phage infection characteristic of a given phage/host system, have been measured for a wide variety of lactic acid bacteria. However, most studies to date were conducted in optimal growth conditions of host bacteria and did not consider variations due to changes in external factors. In this work, we determined the effect of temperature, pH, and starvation on kinetic parameters of phages infecting Lactobacillus paracasei, Lactobacillus plantarum, and Leuconostoc mesenteroides. For kinetics assessment, one-step growth curves were carried out in MRS broth at optimal conditions (control), lower temperature, pH 6.0 and 5.0 (MRS6 and MRS5, respectively), or in medium lacking carbon (MRSN) or nitrogen (MRSC) sources. Phage infection was progressively impaired as environmental conditions were modified from optimal. At lower temperature or pH, infection was delayed, as perceived by longer latent and burst times. Burst size, however, was lower, equal or higher than for controls, but this effect was highly dependent on the particular phage–host system studied. Phage infection was strongly inhibited in MRSC, but only mildly impaired in MRSN. Nevertheless, growth of all the bacterial strains tested was severely compromised by starvation, without significant differences between MRSC and MRSN, indicating that nitrogen compounds are specifically required for a successful phage infection, beyond their influence on bacterial growth.
Food and Environmental Virology – Springer Journals
Published: Apr 8, 2017
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera