Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases

Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy... An interplay between cellulases is fundamental in biomass saccharification. Here, the synergistic action of Trichoderma harzianum Cel7A and Cel7B on two cellulosic substrates: bacterial cellulose (BC) and a much more heterogeneous filter paper (FP) was investigated by determining their saccharification yields and by analyzing both the released soluble products and the insoluble reducing ends formed in the process. Furthermore, morphological changes of the substrates were evaluated using scanning electron microscopy. Glycoside hydrolase family 7 (GH7) enzymes introduce uniform changes in BC, whereas in FP they preferentially consume thin microfibrils rather than thicker paper fibers. Thus, the size effect, which leads to a smaller surface area per unit of substrate mass for thicker fibers, seems to play a crucial role in higher enzymatic hydrolysis efficiency of BC as compared to FP. These results demonstrate that the morphology-dependent effects could be essential for the industrial breakdown of cellulose-rich plant biomass. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cellulose Springer Journals

Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases

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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Chemistry; Bioorganic Chemistry; Physical Chemistry; Organic Chemistry; Polymer Sciences; Ceramics, Glass, Composites, Natural Materials; Sustainable Development
ISSN
0969-0239
eISSN
1572-882X
D.O.I.
10.1007/s10570-018-1700-z
Publisher site
See Article on Publisher Site

Abstract

An interplay between cellulases is fundamental in biomass saccharification. Here, the synergistic action of Trichoderma harzianum Cel7A and Cel7B on two cellulosic substrates: bacterial cellulose (BC) and a much more heterogeneous filter paper (FP) was investigated by determining their saccharification yields and by analyzing both the released soluble products and the insoluble reducing ends formed in the process. Furthermore, morphological changes of the substrates were evaluated using scanning electron microscopy. Glycoside hydrolase family 7 (GH7) enzymes introduce uniform changes in BC, whereas in FP they preferentially consume thin microfibrils rather than thicker paper fibers. Thus, the size effect, which leads to a smaller surface area per unit of substrate mass for thicker fibers, seems to play a crucial role in higher enzymatic hydrolysis efficiency of BC as compared to FP. These results demonstrate that the morphology-dependent effects could be essential for the industrial breakdown of cellulose-rich plant biomass.

Journal

CelluloseSpringer Journals

Published: Feb 12, 2018

References

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