Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu salmon Oncorhynchus masou

Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu... The central nervous system of fish has a high ability to produce new nerve cells in the adult organism in certain neurogenic areas of the brain throughout life. Analysis of proliferative activity and the ability to neuron differentiation was performed in cultured cells of the brain and spinal cord of the juvenile masu salmon Oncorhynchus masou. Proliferating cell nuclear antigen (PCNA) was used as a proliferative marker, while a marker of neuronal differentiation, the neuron protein HuCD, detected neurons. The results showed that cell proliferation occurs mainly in the suspension cell fraction. In monolayer, only a few cells were found to express PCNA. The results of immunocytochemical analysis allow us to conclude that proliferative activity in primary cultures from the O. masou brain is mainly connected with the suspension fraction of small cells. In contrast, the presence of the cells expressing cystathionine β-synthase, a marker of H2S synthesis, and the cells expressing PCNA in the monolayer, may indicate the possible participation of H2S in proliferative activity of neurons in primary cultures. The data obtained suggest that the hydrogen sulphide can be involved in the process of neuronal differentiation; however, the nature of its participation remains largely unknown. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu salmon Oncorhynchus masou

Loading next page...
 
/lp/springer_journal/cell-proliferation-and-differentiation-in-primary-cultures-of-the-54Bvg2O3Da
Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Freshwater & Marine Ecology
ISSN
1063-0740
eISSN
1608-3377
D.O.I.
10.1134/S1063074015060115
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial