Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu salmon Oncorhynchus masou

Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu... The central nervous system of fish has a high ability to produce new nerve cells in the adult organism in certain neurogenic areas of the brain throughout life. Analysis of proliferative activity and the ability to neuron differentiation was performed in cultured cells of the brain and spinal cord of the juvenile masu salmon Oncorhynchus masou. Proliferating cell nuclear antigen (PCNA) was used as a proliferative marker, while a marker of neuronal differentiation, the neuron protein HuCD, detected neurons. The results showed that cell proliferation occurs mainly in the suspension cell fraction. In monolayer, only a few cells were found to express PCNA. The results of immunocytochemical analysis allow us to conclude that proliferative activity in primary cultures from the O. masou brain is mainly connected with the suspension fraction of small cells. In contrast, the presence of the cells expressing cystathionine β-synthase, a marker of H2S synthesis, and the cells expressing PCNA in the monolayer, may indicate the possible participation of H2S in proliferative activity of neurons in primary cultures. The data obtained suggest that the hydrogen sulphide can be involved in the process of neuronal differentiation; however, the nature of its participation remains largely unknown. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Marine Biology Springer Journals

Cell proliferation and differentiation in primary cultures of the juvenile brain of the masu salmon Oncorhynchus masou

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Pleiades Publishing
Copyright © 2015 by Pleiades Publishing, Ltd.
Life Sciences; Freshwater & Marine Ecology
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