cDNA cloning of luteinizing hormone subunits from brushtail possum and red kangaroo

cDNA cloning of luteinizing hormone subunits from brushtail possum and red kangaroo Luteinizing hormone (LH) plays an important role in the reproductive cycles of all mammals. There is a large amount of both nucleotide and amino acid sequence data available for LH from eutherian mammals, but little is known about the primary structure of LH in marsupials. We have used consensus PCR primers to generate specific probes for screening pituitary cDNA libraries and report the cloning of the cDNAs encoding the α-subunit of LH (also shared by a number of other glycoprotein hormones) and the LH-specific β-subunit, from the common brushtail possum, Trichosurus vulpecula, and the red kangaroo, Macropus rufus. Southern blotting experiments indicated that both genes are probably present as single copies. Comparison of the deduced marsupial protein sequences with homologous sequences from other vertebrates revealed a high degree of conservation, especially for the α-subunit. These sequences represent the first complete primary structures for a marsupial glycoprotein hormone to have been elucidated. Mammalian Genome Springer Journals

cDNA cloning of luteinizing hormone subunits from brushtail possum and red kangaroo

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Copyright © 1998 by Springer-Verlag New York Inc.
Life Sciences; Cell Biology; Animal Genetics and Genomics; Human Genetics
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