cDNA cloning and analysis of polymorphism of GFI1B gene in GuiZhou white goat

cDNA cloning and analysis of polymorphism of GFI1B gene in GuiZhou white goat This study mainly aimed to research the CDS sequence (coding sequence), polymorphisms of exon 1, exon 2 and part of exon 8 of GFI1B in GuiZhou white goat of indigenous Chinese goat. The cDNA of GFI1B was analysed through bioinformatics which was obtained by the reverse transcription PCR (RT-PCR). The polymorphisms of GFI1B gene exons region was identified whose association with growth traits was analysed by GuiZhou white goat. The results revealed that the cDNA of GFI1B which contained a complete open reading frame (ORF) was 996 bp in full length. The accession numbers on GenBank was JX627316. One new mutation sites of C347A had been determined in the eighth intron. Our results could lay the foundation for the further investigation on the role of GFI1B gene in goat growth traits. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

cDNA cloning and analysis of polymorphism of GFI1B gene in GuiZhou white goat

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795413110045
Publisher site
See Article on Publisher Site

Abstract

This study mainly aimed to research the CDS sequence (coding sequence), polymorphisms of exon 1, exon 2 and part of exon 8 of GFI1B in GuiZhou white goat of indigenous Chinese goat. The cDNA of GFI1B was analysed through bioinformatics which was obtained by the reverse transcription PCR (RT-PCR). The polymorphisms of GFI1B gene exons region was identified whose association with growth traits was analysed by GuiZhou white goat. The results revealed that the cDNA of GFI1B which contained a complete open reading frame (ORF) was 996 bp in full length. The accession numbers on GenBank was JX627316. One new mutation sites of C347A had been determined in the eighth intron. Our results could lay the foundation for the further investigation on the role of GFI1B gene in goat growth traits.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Nov 7, 2013

References

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