CbCBF from Capsella bursa-pastoris enhances cold tolerance and restrains growth in Nicotiana tabacum by antagonizing with gibberellin and affecting cell cycle signaling

CbCBF from Capsella bursa-pastoris enhances cold tolerance and restrains growth in Nicotiana... Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Capsella bursa-pastoris named CbCBF, which was responsive to chilling temperatures. Here, we show that CbCBF expression is obviously responsive to chilling, freezing, abscisic acid, gibberellic acid (GA), indoleacetic acid or methyl jasmonate treatments and that the CbCBF:GFP fusion protein was localized to the nucleus. In addition, CbCBF overexpression conferred to the cold-sensitive tobacco plants enhanced tolerance to chilling and freezing, as well as dwarfism and delayed flowering. The leaf cells of CbCBF overexpression tobacco lines attained smaller sizes and underwent delayed cell division with reduced expression of cyclin D genes. The dwarfism of CbCBF transformants can be partially restored by GA application. Consistently, CbCBF overexpression reduced the bioactive gibberellin contents and disturbed the expression of gibberellin metabolic genes in tobacco. Meanwhile, cold induced CbCBF expression and cold tolerance in C. bursa-pastoris are reduced by GA. We conclude that CbCBF confers cold resistance and growth inhibition to tobacco cells by interacting with gibberellin and cell cycle pathways, likely through activation of downstream target genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

CbCBF from Capsella bursa-pastoris enhances cold tolerance and restrains growth in Nicotiana tabacum by antagonizing with gibberellin and affecting cell cycle signaling

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Publisher
Springer Netherlands
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-014-0181-1
Publisher site
See Article on Publisher Site

Abstract

Plant cells respond to cold stress via a regulatory mechanism leading to enhanced cold acclimation accompanied by growth retardation. The C-repeat binding factor (CBF) signaling pathway is essential for cold response of flowering plants. Our previously study documented a novel CBF-like gene from the cold-tolerant Capsella bursa-pastoris named CbCBF, which was responsive to chilling temperatures. Here, we show that CbCBF expression is obviously responsive to chilling, freezing, abscisic acid, gibberellic acid (GA), indoleacetic acid or methyl jasmonate treatments and that the CbCBF:GFP fusion protein was localized to the nucleus. In addition, CbCBF overexpression conferred to the cold-sensitive tobacco plants enhanced tolerance to chilling and freezing, as well as dwarfism and delayed flowering. The leaf cells of CbCBF overexpression tobacco lines attained smaller sizes and underwent delayed cell division with reduced expression of cyclin D genes. The dwarfism of CbCBF transformants can be partially restored by GA application. Consistently, CbCBF overexpression reduced the bioactive gibberellin contents and disturbed the expression of gibberellin metabolic genes in tobacco. Meanwhile, cold induced CbCBF expression and cold tolerance in C. bursa-pastoris are reduced by GA. We conclude that CbCBF confers cold resistance and growth inhibition to tobacco cells by interacting with gibberellin and cell cycle pathways, likely through activation of downstream target genes.

Journal

Plant Molecular BiologySpringer Journals

Published: Feb 15, 2014

References

  • The cold-inducible CBF1 factor-dependent signaling pathway modulates the accumulation of the growth-repressing DELLA proteins via its effect on gibberellin metabolism
    Achard, P; Gong, F; Cheminant, S; Alioua, M; Hedden, P; Genschik, P
  • Cell cycle-dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells
    Criqui, MC; Parmentier, Y; Derevier, A; Shen, WH; Dong, AW; Genschik, P
  • OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression
    Dubouzet, JG; Sakuma, Y; Ito, Y; Kasuga, M; Dubouzet, EG; Miura, S; Seki, M; Shinozaki, K; Yamaguchi-Shinozaki, K
  • Regulation and characterization of four CBF transcription factors from Brassica napus
    Gao, MJ; Allard, G; Byass, L; Flanagan, AM; Singh, J
  • Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis
    Haake, V; Cook, D; Riechmann, JL; Pineda, O; Thomashow, MF; Zhang, JZ
  • Tomato plants ectopically expressing Arabidopsis CBF1 show enhanced resistance to water deficit stress
    Hsieh, TH; Lee, JT; Charng, YY; Chan, MT
  • MAFFT multiple sequence alignment software version 7: improvements in performance and usability
    Katoh, K; Standley, DM
  • Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance
    Kitashiba, H; Ishizaka, T; Isuzugawa, K; Nishimura, K; Suzuki, T

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