Carrageenanolytic enzymes from marine bacteria associated with the red alga Tichocarpus crinitus

Carrageenanolytic enzymes from marine bacteria associated with the red alga Tichocarpus crinitus One hundred six bacterial strains (106 strains) were isolated from the Pacific red alga Tichocarpus crinitus, collected from the Troitsa Bay of Gulf of Peter the Great (Sea of Japan, Russia), and studied on their ability to degrade different samples of carrageenan as substrates. Some of the studied strains exhibited hydrolytic activity selectively to a total polysaccharide (kappa and lambda) from Chondrus armatus, other strains, to κ/β-carrageenan from T. crinitus, which did not possess the same effect to a total polysaccharide from T. crinitus. The strains with the highest enzyme activity (28 strains) were identified using 16S rRNA gene sequence techniques and classified to the phyla Bacteroidetes and Proteobacteria. Most (75%) of the studied carrageenase producers belong to phylum Bacteroidetes (21 strains). These strains were related with 7 phylotypes of the genera Aquimarina, Cellulophaga, Maribacter, and Zobellia (family Flavobacteriaceae of the class Flavobacteriia). Cellulophaga strains were the dominant group (35.7% of total) and closely related to species C. baltica and C. lytica with 99.8 and 99.9% sequence similarity, respectively. The remaining strains (7 strains) were represented by members of the genera Altererythrobacter (the family Erythrobacteraceae), Phaeobacter, Sulfitobacter, Tateyamaria (the family Rhodobacteraceae), and Sphingomonas (the family Sphingobacteriaceae) of the class Alphaproteobacteria and Psychrobacter (the family Moraxellaceae) and Vibrio (the family Vibrionaceae) of the class Gammaproteobacteria. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Phycology Springer Journals

Carrageenanolytic enzymes from marine bacteria associated with the red alga Tichocarpus crinitus

Loading next page...
 
/lp/springer_journal/carrageenanolytic-enzymes-from-marine-bacteria-associated-with-the-red-sTqFOB5VFj
Publisher
Springer Journals
Copyright
Copyright © 2017 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Plant Sciences; Freshwater & Marine Ecology; Plant Physiology; Ecology
ISSN
0921-8971
eISSN
1573-5176
D.O.I.
10.1007/s10811-017-1355-4
Publisher site
See Article on Publisher Site

Abstract

One hundred six bacterial strains (106 strains) were isolated from the Pacific red alga Tichocarpus crinitus, collected from the Troitsa Bay of Gulf of Peter the Great (Sea of Japan, Russia), and studied on their ability to degrade different samples of carrageenan as substrates. Some of the studied strains exhibited hydrolytic activity selectively to a total polysaccharide (kappa and lambda) from Chondrus armatus, other strains, to κ/β-carrageenan from T. crinitus, which did not possess the same effect to a total polysaccharide from T. crinitus. The strains with the highest enzyme activity (28 strains) were identified using 16S rRNA gene sequence techniques and classified to the phyla Bacteroidetes and Proteobacteria. Most (75%) of the studied carrageenase producers belong to phylum Bacteroidetes (21 strains). These strains were related with 7 phylotypes of the genera Aquimarina, Cellulophaga, Maribacter, and Zobellia (family Flavobacteriaceae of the class Flavobacteriia). Cellulophaga strains were the dominant group (35.7% of total) and closely related to species C. baltica and C. lytica with 99.8 and 99.9% sequence similarity, respectively. The remaining strains (7 strains) were represented by members of the genera Altererythrobacter (the family Erythrobacteraceae), Phaeobacter, Sulfitobacter, Tateyamaria (the family Rhodobacteraceae), and Sphingomonas (the family Sphingobacteriaceae) of the class Alphaproteobacteria and Psychrobacter (the family Moraxellaceae) and Vibrio (the family Vibrionaceae) of the class Gammaproteobacteria.

Journal

Journal of Applied PhycologySpringer Journals

Published: Dec 17, 2017

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off