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L. Hermida, R. Rodríguez, L. Lazo, C. López, G. Márquez, R. Páez, C. Suárez, R. Espinosa, J. García, G. Guzmán, G. Guillén (2002)
A recombinant envelope protein from Dengue virus purified by IMAC is bioequivalent with its immune-affinity chromatography purified counterpart.Journal of biotechnology, 94 2
C. Delenda, M. Frenkiel, V. Deubel (2005)
Protective efficacy in mice of a secreted form of recombinant dengue-2 virus envelope protein produced in baculovirus infected insect cellsArchives of Virology, 139
A. Mazzei (1998)
[Molecular biology of dengue virus].Acta cientifica venezolana, 49 Suppl 1
(2003)
Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induces neutralizing antibodies and provides partial protection from viral challenge in Macaca fascicularis
C. Delenda, I. Staropoli, M. Frenkiel, L. Cabanié, V. Deubel (1994)
Analysis of C-terminally truncated dengue 2 and dengue 3 virus envelope glycoproteins: processing in insect cells and immunogenic properties in mice.The Journal of general virology, 75 ( Pt 7)
P. Mason, M. Zügel, A. Semproni, M. Fournier, T. Mason (1990)
The antigenic structure of dengue type 1 virus envelope and NS1 proteins expressed in Escherichia coli.The Journal of general virology, 71 ( Pt 9)
M. Simmons, Gerald Murphy, Curtis Hayes (2001)
Short report: Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccine.The American journal of tropical medicine and hygiene, 65 2
U. Laemmli (1970)
Cleavage of structural proteins duringNature
V. Deubel, R. Kinney, J. Esposito, Cropp Cb, A. Vorndam, T. Monath, D. Trent (1988)
Dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against dengue.The Journal of general virology, 69 ( Pt 8)
(1993)
Rapid transformation of non Sacharomyces yeast by electroporation
H. Bisht, Dipti Chugh, S. Swaminathan, N. Khanna (2001)
Expression and purification of Dengue virus type 2 envelope protein as a fusion with hepatitis B surface antigen in Pichia pastoris.Protein expression and purification, 23 1
(1989)
Use of dengue structural proteins and non structural protein NS1 by recombinant baculovirus for immunization against dengue virus infection
Vincent Deubel, Manola Laille, Jean-Philippe Hugnot, Eliane Chungue, Jean-Luc Guesdon, M. Drouet, Sylviane Bassot, Daniele Chevrier (1990)
Identification of dengue sequences by genomic amplification: rapid diagnosis of dengue virus serotypes in peripheral blood.Journal of virological methods, 30 1
H. Fujita, H. Sumiyoshi, C. Mori, S. Manabe, M. Takagi, I. Yoshida, K. Morita, I. Fuke, K. Fukai, A. Igarashi (1987)
Studies in the development of Japanese encephalitis vaccine: expression of virus envelope glycoprotein V3 (E) gene in yeast.Bulletin of the World Health Organization, 65 3
R. Men, Michael Bray, Ching-Juh Lai (1991)
Carboxy-terminally truncated dengue virus envelope glycoproteins expressed on the cell surface and secreted extracellularly exhibit increased immunogenicity in miceJournal of Virology, 65
Edward Hayes, Duane Gubler (1992)
Dengue and dengue hemorrhagic fever.The Pediatric infectious disease journal, 11 4
A. Mazzei (1998)
Molecular biology of dengue virus
M. Guzmán, R. Rodríguez, R. Rodríguez, L. Hermida, M. Álvarez, L. Lazo, M. Muné, D. Rosario, K. Valdés, S. Vázquez, R. Martínez, T. Serrano, Jorge Paez, R. Espinosa, Tania Pumariega, G. Guillén (2003)
Induction of neutralizing antibodies and partial protection from viral challenge in Macaca fascicularis immunized with recombinant dengue 4 virus envelope glycoprotein expressed in Pichia pastoris.The American journal of tropical medicine and hygiene, 69 2
D. Clarke, J. Casals (1958)
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.The American journal of tropical medicine and hygiene, 7 5
(2001)
Antibody responses of mice immunized with a tetravalent dengue recombinant protein subunit vaccine.Am
(2000)
Process for the expression of genes of the Dengue viruses
S. Halstead (1988)
Pathogenesis of dengue: challenges to molecular biology.Science, 239 4839
R. Sugrue, Jianlin Fu, Josephine Howe, Y. Chan (1997)
Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles.The Journal of general virology, 78 ( Pt 8)
David Morens, Scott Halstead, Patricia Repik, Ravithat Putvatana, '. Raybourne (1985)
Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralizationJournal of Clinical Microbiology, 22
M. Simmons, William Nelson, Shuenn-Jue Wu, Curtis Hayes (1998)
Evaluation of the protective efficacy of a recombinant dengue envelope B domain fusion protein against dengue 2 virus infection in mice.The American journal of tropical medicine and hygiene, 58 5
W. Burnette (1981)
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.Analytical biochemistry, 112 2
R. Feighny, J. Burrous, R. Putnak (1994)
Dengue type-2 virus envelope protein made using recombinant baculovirus protects mice against virus challenge.The American journal of tropical medicine and hygiene, 50 3
We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris . The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001).
Archives of Virology – Springer Journals
Published: Nov 1, 2003
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