Russian Journal of Applied Chemistry, 2009, Vol. 82, No. 2, pp. 312−316.
Pleiades Publishing, Ltd., 2009.
Original Russian Text
V.G. Berezkin, L.A. Onuchak, E.N. Evtyugina, 2009, published in Zhurnal Prikladnoi Khimii, 2009, Vol. 82, No. 2, pp. 317−321.
AND INDUSTRIAL ORGANIC CHEMISTRY
Capillary Thin-Layer Chromatography of Antibacterial
V. G. Berezkin, L. A. Onuchak, and E. N. Evtyugina
Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Moscow, Russia
Samara State University, State Educational Institution, Samara, Russia
Received January 16, 2008
Abstract—A new version of thin-layer chromatography, capillary thin-layer chromatography, which is similar
to traditional planar chromatography in the idea of detection of sorbate bands and to high-performance column
chromatography in the implementation of the chromatographic process (in a thin capillary packed with a sorbent),
was developed. The chromatographic characteristics of antibacterial nitrofuran derivatives under the conditions of
planar and capillary thin-layer chromatography were studied and compared from the viewpoint of performance,
reproducibility, and consumption of the sorbent and eluent.
Thin-layer chromatography (TLC) is widely used
in medicine, biology, and pharmacy (in drug quality
control), and also in various branches of industry and
in scientiﬁ c research . Wide use of TLC is associated
with the fact that the method is prompt, sensitive, simple,
readily accessible, and cheap. However, the method
has also certain drawbacks such as low performance,
the necessity of using special chambers saturated
with mobile phase vapor, and, as a consequence, poor
reproducibility of the chromatographic retention indices
In this study we suggested a new version of TLC,
so-called capillary thin-layer chromatography (CTLC),
which is similar to traditional planar TLC in the
driving force of the liquid phase ﬂ ow and in the idea
of detection of sorbate bands and to high-performance
column chromatography in the implementation of the
chromatographic process (in a thin capillary packed with
a sorbent). In CTLC, the motion and smearing of sorbate
bands occur in a very thin sorbent layer (≤0.5 mm) in the
The new version of TLC was implemented with
colored compounds, antibacterial nitrofuran derivatives,
as example. Nitrofuran derivatives are used in medicine
for treating infectious diseases .
As investigation objects we used drugs based on
nitrofuran derivatives: Furagin, Furadonin, Furazolidone,
Furacillin, and Ercefuryl. The structural formulas and
physicochemical properties of the compounds are given
in Table 1.
Samples of substances for the experiments were
prepared from drug forms. A tablet was ﬁ nely divided, and
the target substance was extracted with 2 ml of acetonitrile
for 0.5 h. The concentrations of the substances extracted
from tablets were 0.025 g cm
For traditional ascending TLC, we used Sorbfil
PTSKh-AF-V-UF plates (silica gel STKh-1VE as sorbent,
dp = 8–12 μm, aluminum support, UV 254 nm) of size
10 × 10 cm, produced by Sorbpolimer Private Company
(Krasnodar, Russia). Chromatography was performed in
the ascending ﬂ ow in chambers saturated with eluent vapor.
The experiments were performed at room temperature. The
eluent path length was 7.5 cm.
For capillary TLC we used capillaries made of melted
quartz (without stationary phase on the internal surface),
0.53 mm i.d., produced by Phenomenex (USA) and sold
by Akvilon (Moscow). The external polymeric coating of
the capillaries was removed by keeping them in acetonitrile