ISSN 1070-4272, Russian Journal of Applied Chemistry, 2006, Vol. 79, No. 10, pp. 1634!1638. + Pleiades Publishing, Inc., 2006.
Original Russian Text + T.G. Dzherayan, A.V. Rudnev, V.P. Morgalyuk, 2006, published in Zhurnal Prikladnoi Khimii, 2006, Vol. 79, No. 10,
AND POLYMERIC MATERIALS
Capillary Electrophoretic Study of Synthesis
T. G. Dzherayan, A. V. Rudnev, and V. P. Morgalyuk
Vernadsky Institute of Geochemistry and Analytical Chemistry,
Russian Academy of Sciences, Moscow, Russia
Received December 21, 2005; in final form, April 2006
Abstract-Synthesis of polyhexamethyleneguanidine hydrochloride was studied by capillary electrophoresis.
A procedure for determining the reaction components was developed.
Presently, control of microbial pathogens (human
and animal infectious agents) is a very topical prob-
lem. It has been demonstrated that water-soluble salts
of the high-molecular-weight polycationic compound,
polyhexamethyleneguanidine hydrochloride (PHMG),
show high biocidal activity against pathogenic micro-
organisms and low toxicity [1, 2]. However, their
application as biocidal agents, especially in medical
practice, requires a high quality of the polymer,
since admixtures of the initial monomers of guani-
dine hydrochloride (GHC) and hexamethylenediamine
(HMDA) impart toxic and allergic properties to
the product. It has also been demonstrated  that,
in some cases, the biocidal characteristics of the pro-
duct are dependent on the preparation procedure.
Therefore, to control the course of the synthesis and
to elucidate the sources of the biocidal activity loss,
it was necessary to develop a procedure for prompt
analysis of the product and to determine the reaction
It is known that capillary electrophoresis (CE) is
successfully used for separation and determination of
such high-molecular-weight compounds as oligonu-
cleotides, oligopeptides, and oligisaccharides [3, 4].
For example, CE has been used to separate polycat-
ionic dendrimers of polyaminoamides with various
charge/molecular weight ratios .
The goals of this study included were to optimize
the conditions of separation and determination of
polymeric forms of PHMG (properties of the sam-
ples varied, depending on the synthetic conditions)
and starting reagents used for its synthesis (GHC
and HMDA), determine the reaction mechanism of
synthesis, and searching for the ways to monitor
the course of the synthesis.
As samples for analysis we used PHMG, GHC,
HMDA, and also 1,6-diguanidinohexane dihydrochlo-
NH . HCl
NH . HCl
NH . HCl
NH . HCl
Buffer solutions with pH 6.86 and 9.18 were pre-
pared from NaH
. 10 H
standard titrants, using double-distilled water.
For analysis we used a Kapel-105 capillary elec-
trophoresis system (Lumex, St. Petersburg, Russia)
equipped with a variable-wavelength UV detector
(wavelength range 1903380 nm) and hydraulic sam-
ple injection system. We used a quartz capillary
Samples were synthesized and purified at the Institute of
Ecotechnological Problems, Moscow, Russia.