Appl Biochem Biotechnol https://doi.org/10.1007/s12010-018-2799-y Cantilever Functionalization Using Peroxidase Extract of Low Cost for Glyphosate Detection 1 1 Daniela Kunkel Muenchen & Janine Martinazzo & 1 1 Alexandra Nava Brezolin & Alana Marie de Cezaro & 1 1 Aline Andressa Rigo & Mateus Nava Mezarroba & 1 2 Alexandra Manzoli & Fábio de Lima Leite & 1 1 Juliana Steffens & Clarice Steffens Received: 14 February 2018 /Accepted: 28 May 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract A cantilever nanobiosensor functionalized with vegetable source of peroxidase was developed as an innovative way for glyphosate herbicide detection over a wide concentration −1 range (0.01 to 10 mg L ) using atomic force microscopy (AFM) technique. The extract obtained from zucchini (Cucurbita pepo source of peroxidase), with high enzymatic activity and stability has been used as bio-recognition element to develop a nanobiosensor. The polarization-modulated reflection absorption infrared spectroscopy (PM-RAIRS) demonstrat- ed the deposition of enzyme on cantilever surface using self-assembled monolayers (SAM) by the presence of the amide I and II bands. The detection mechanism of glyphosate was based on the changes in surface tension caused by the analyte adsorption, resulting in a conformational change in the enzyme
Applied Biochemistry and Biotechnology – Springer Journals
Published: Jun 4, 2018
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