Canna paniculata as natural host of canna yellow streak
virus in Brazil
M. A. V. Alexandre
L. M. L. Duarte
A. L. R. Chaves
A. F. Ramos
E. W. Kitajima
Received: 23 May 2017 /Accepted: 27 July 2017 /Published online: 10 August 2017
Australasian Plant Pathology Society Inc. 2017
Abstract Canna yellow streak virus (CaYSV) was identified
on Canna paniculata with foliar mosaic symptoms from
Piracicaba municipality (São Paulo state, Brazil) by transmis-
sion electron microscopy, biological, serological and molecular
assays. Pairwise analyses of nucleotide and amino acid se-
quences (potyvirus cylindrical inclusion region) with homolo-
gous sequences revealed the highest identity (88.6 and 97.8%)
with CaYSV. This is the first detection of CaYSV in Brazil.
Cannaceae is of neotropical origin, with native species from
the Southern United States to Northern Argentina (Maas-Van
de Kamer and Maas 2008). Of the 217 scientific plant names
cited by the Plant List (2013), only 12 are accepted as species
names. In Brazil, the occurrence of four native Canna species
(C. glauca, C. indica, C. paniculata and C. pedunculata)has
been reported, all concentrated in the South and Southeast
(Souza and Lorenzi 2012).
Few viruses have been reported in canna plants worldwide;
these include Canna yellow mottle virus (CaYMV), a
badnavirus, cucumber mosaic virus (CMV) and tomato
aspermy virus (TAV), cucumovirus, as well as bean yellow
mosaic virus (BYMV) and sugarcane mosaic virus (SCMV),
both potyviruses (Monger et al. 2007; Marino et al. 2008;
Rajakaruna et al. 2014;Tangetal.2016). Another potyvirus,
Canna yellow streak virus (CaYSV), was reported in the
United Kingdom in 2007, and other countries (Monger et al.
2007; Rajakaruna et al. 2014; Chauhan et al. 2015). Although
the canna lily is commonly found in nature as well as in public
and private gardens in Brazil, to date there has been no report-
ed occurrence of viral diseases in these plants. As such, the
aim of this study was to identify and characterize virus(es)
associated with mosaic symptoms in Canna paniculata (sin.
C. brasiliensis, C. denudata, Distemon kunzei, D. miniatus)
leaf samples (Fig. 1) from a public garden in the municipality
of Piracicaba, São Paulo state.
Elongated flexuous particles were observed by electron
microscopy in extracts from negatively stained symptomatic
canna plants (Canna Br01). Ultra-thin sections showed the
presence of type II lamellar inclusions, according to
Edwardson and Christie’s(1996) classification, suggesting
the sample was infected with a potyvirus.
Indirect ELISAwith antisera against BYMVand CMV, and
DAS-ELISA with Potyvirus group and banana bunchy top
virus (BBTV, Babuvirus) – commercial antisera (AGDIA),
exhibited negative results. BBTV was included because it is
considered a quarantine pest in Brazil, and C. indica may be
an alternative host of this virus even though it is closely asso-
ciated with Musa sp. (Pinili et al.
684 bp fragment corresponding to the potyviral CI region
was produced from Canna-Br01 after total RNA extraction with
Trizol (Life Technologies Ambion) and RT-PCR using gene-
specific primers for the cylindrical inclusion region (CI) of the
potyvirus (Ha et al. 2008). Forward and reverse sequencing of
the amplicon was performed in an automated DNA sequencer,
followed by BLAST analysis and alignment with those
* M. A. V. Alexandre
Laboratório de Fitovirologia Fisiopatológica, Instituto Biológico, Av.
Conselheiro Rodrigues Alves, 1252, CEP 04014-900 São Paulo, SP,
Laboratório de Bioquímica Fitopatológica, Biológico, Av.
Conselheiro Rodrigues Alves, 1252, CEP 040140-900 São
Paulo, SP, Brazil
Escola Superior de Agricultura Luiz de Queiróz, USP, CP 09, CEP
13418-900 Piracicaba, SP, Brazil
Australasian Plant Dis. Notes (2017) 12: 38