Callusogenesis and somatic embryogenesis induction in hybrid embryos from the seeds of Pinus sibirica

Callusogenesis and somatic embryogenesis induction in hybrid embryos from the seeds of Pinus... The results of long-term work on the induction of somatic embryogenesis in Siberian pine (Pinus sibirica Du Tour) growing in a natural stand of trees and in clone grafting plantation located in the Western Sayan are shown. Controlled pollination of the clones of Siberian pine had a positive influence on the state of callus cultures. The cytological analysis of embryonal-suspensor mass made it possible to identify embryological structures morphologically close to zygotic embryos at early developmental stages; as a result, the callus tissue was recognized embryogenic. We revealed donor plants (clones), whose zygotic embryos in vitro can serve as a source of embryogenic callus tissue. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Callusogenesis and somatic embryogenesis induction in hybrid embryos from the seeds of Pinus sibirica

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Publisher
Springer US
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714020162
Publisher site
See Article on Publisher Site

Abstract

The results of long-term work on the induction of somatic embryogenesis in Siberian pine (Pinus sibirica Du Tour) growing in a natural stand of trees and in clone grafting plantation located in the Western Sayan are shown. Controlled pollination of the clones of Siberian pine had a positive influence on the state of callus cultures. The cytological analysis of embryonal-suspensor mass made it possible to identify embryological structures morphologically close to zygotic embryos at early developmental stages; as a result, the callus tissue was recognized embryogenic. We revealed donor plants (clones), whose zygotic embryos in vitro can serve as a source of embryogenic callus tissue.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Mar 7, 2014

References

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