Cadmium effect on vacuolar H+-ATPase gene expression in the roots of barley seedlings of different age

Cadmium effect on vacuolar H+-ATPase gene expression in the roots of barley seedlings of... Expression of two genes (HvVHA E and HvVHA c) of vacuolar H+-ATPase was studied in the cells of barley (Hordeum vulgare L., cv. Zazerskii 85) roots in seedlings of different age in the presence of cadmium (100 μM). Three-day-old seedlings were kept for four days on cadmium solution, which caused suppression of root growth accompanied by an increase in HvVHA E gene expression. In this case, the content of reduced glutathione (GSH) decreased. When seven-day-old seedlings were placed on cadmium solution and kept there for four days, the content of metal in the root was higher, but its growth was not suppressed and expression of both studied genes markedly increased. The content of GSH also apparently increased, ensuring the maintenance of high enzyme activity. A comparison of gene expression encoding two subunits of vacuolar H+-ATPase and cadmium resistance of the seedlings of different age showed that the enzyme participates in the mechanism of the improvement of barley tolerance to this metal. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Cadmium effect on vacuolar H+-ATPase gene expression in the roots of barley seedlings of different age

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Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443713010032
Publisher site
See Article on Publisher Site

Abstract

Expression of two genes (HvVHA E and HvVHA c) of vacuolar H+-ATPase was studied in the cells of barley (Hordeum vulgare L., cv. Zazerskii 85) roots in seedlings of different age in the presence of cadmium (100 μM). Three-day-old seedlings were kept for four days on cadmium solution, which caused suppression of root growth accompanied by an increase in HvVHA E gene expression. In this case, the content of reduced glutathione (GSH) decreased. When seven-day-old seedlings were placed on cadmium solution and kept there for four days, the content of metal in the root was higher, but its growth was not suppressed and expression of both studied genes markedly increased. The content of GSH also apparently increased, ensuring the maintenance of high enzyme activity. A comparison of gene expression encoding two subunits of vacuolar H+-ATPase and cadmium resistance of the seedlings of different age showed that the enzyme participates in the mechanism of the improvement of barley tolerance to this metal.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Dec 28, 2012

References

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