Ca2+-Induced Effect on Mechanical Properties of Sulfatide-Incorporated Vesicles

Ca2+-Induced Effect on Mechanical Properties of Sulfatide-Incorporated Vesicles The Ca2+-induced effect on the nanomechanical properties of vesicles prepared at a different ratio of dipalmitoylphosphatidylcholine (DPPC)/sulfatide was studied using atomic force microscope (AFM) on a mica surface. Vesicles were prepared by extrusion and adsorbed on the mica surface. The forces, measured between an AFM tip and the vesicle, showed that the breakthrough of the tip into the vesicles occurred two times. Force data prior to the first breakthrough were fitted well with the Hertzian model to estimate Young’s modulus and bending modulus of the vesicles. Sulfatide incorporation led to a decrease of around 90% in Young’s modulus and bending modulus of the vesicles due to the hydration of the headgroups, while the addition of Ca2+ induced dehydration to recover the properties. The change of the physical properties seems to be attributed to the headgroup packing order of the vesicles, which is determined by the interference with the hydration shell. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Membrane Biology Springer Journals

Ca2+-Induced Effect on Mechanical Properties of Sulfatide-Incorporated Vesicles

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Publisher
Springer-Verlag
Copyright
Copyright © 2010 by Springer Science+Business Media, LLC
Subject
Life Sciences; Human Physiology ; Biochemistry, general
ISSN
0022-2631
eISSN
1432-1424
D.O.I.
10.1007/s00232-010-9319-5
Publisher site
See Article on Publisher Site

Abstract

The Ca2+-induced effect on the nanomechanical properties of vesicles prepared at a different ratio of dipalmitoylphosphatidylcholine (DPPC)/sulfatide was studied using atomic force microscope (AFM) on a mica surface. Vesicles were prepared by extrusion and adsorbed on the mica surface. The forces, measured between an AFM tip and the vesicle, showed that the breakthrough of the tip into the vesicles occurred two times. Force data prior to the first breakthrough were fitted well with the Hertzian model to estimate Young’s modulus and bending modulus of the vesicles. Sulfatide incorporation led to a decrease of around 90% in Young’s modulus and bending modulus of the vesicles due to the hydration of the headgroups, while the addition of Ca2+ induced dehydration to recover the properties. The change of the physical properties seems to be attributed to the headgroup packing order of the vesicles, which is determined by the interference with the hydration shell.

Journal

The Journal of Membrane BiologySpringer Journals

Published: Nov 19, 2010

References

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